Synaptic Systems - Synapsin1

Cat. No.: 106 011

Amount: 100 µg

Price: $440.00

Cat. No. 106 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 2000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 200 gallery DNA-PAINT: yes EM: yes ELISA: yes (see remarks)
Clone	46.1
Subtype	IgG1
Immunogen	full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop	AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity	Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals. Weaker signal: zebrafish, chicken, other vertebrates. Other species not tested yet.
Specificity	Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Remarks	ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 106 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	106_011.pdf

References for Synapsin1 - 106 011

Polygenic risk for alcohol use disorder affects cellular responses to ethanol exposure in a human microglial cell model.
Li X, Liu J, Boreland AJ, Kapadia S, Zhang S, Stillitano AC, Abbo Y, Clark L, Lai D, Liu Y, Barr PB, et al.
Science advances (2024) 1045: eado5820. 106 011 WB, ICC; tested species: mouse

Adult-onset deactivation of autophagy leads to loss of synapse homeostasis and cognitive impairment, with implications for alzheimer disease.
Grosso Jasutkar H, Wasserlein EM, Ishola A, Litt N, Staniszewski A, Arancio O, Yamamoto A
Autophagy (2024) : 1-16. 106 011 WB; tested species: mouse

The lncRNA Malat1 is trafficked to the cytoplasm as a localized mRNA encoding a small peptide in neurons.
Xiao W, Halabi R, Lin CH, Nazim M, Yeom KH, Black DL
Genes & development (2024) : . 106 011 WB; tested species: mouse

CSF1R-mediated myeloid cell depletion shifts the ratio of motor cortical excitatory to inhibitory neurons in a multiple system atrophy model.
Gauer C, Battis K, Schneider Y, Florio JB, Mante M, Kim HY, Rissman RA, Hoffmann A, Winkler J
Experimental neurology (2024) 374: 114706. 106 011 WB; tested species: mouse

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 106 011 WB; tested species: mouse

Developmental Pb exposure increases AD risk via altered intracellular Ca2+ homeostasis in hiPSC-derived cortical neurons.
Xie J, Wu S, Szadowski H, Min S, Yang Y, Bowman AB, Rochet JC, Freeman JL, Yuan C
The Journal of biological chemistry (2023) : 105023. 106 011 WB, ICC; tested species: human

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.
Goldsmith J, Ordureau A, Harper JW, Holzbaur ELF
Neuron (2022) 1106: 967-976.e8. 106 011 WB; tested species: human

Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 106 011 WB; tested species: mouse

De Novo Missense Variants in SLC32A1 Cause a Developmental and Epileptic Encephalopathy Due to Impaired GABAergic Neurotransmission.
Platzer K, Sticht H, Bupp C, Ganapathi M, Pereira EM, Le Guyader G, Bilan F, Henderson LB, Lemke JR, Taschenberger H, Brose N, et al.
Annals of neurology (2022) : . 106 011 WB; tested species: mouse

Control of protein synthesis and memory by GluN3A-NMDA receptors through inhibition of GIT1/mTORC1 assembly.
Conde-Dusman MJ, Dey PN, Elía-Zudaire Ó, Rabaneda LG, García-Lira C, Grand T, Briz V, Velasco ER, Andero R, Niñerola S, Barco A, et al.
eLife (2021) 10: . 106 011 WB; tested species: rat

The Different Molecular Code in Generation of Dopaminergic Neurons from Astrocytes and Mesenchymal Stem Cells.
Wang N, Ji X, Wu Y, Zhou S, Peng H, Wang J, Yu S, Zhang J
International journal of molecular sciences (2021) 2222: . 106 011 WB; tested species: rat

Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 106 011 WB; tested species: mouse

The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 106 011 WB; tested species: rat

Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 106 011 WB, ICC; tested species: mouse

Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes.
Poston RG, Murphy L, Rejepova A, Ghaninejad-Esfahani M, Segales J, Mulligan K, Saha RN
The Journal of biological chemistry (2020) : . 106 011 WB; tested species: rat

Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
Zhang RS, Liakath-Ali K, Südhof TC
eLife (2020) 9: . 106 011 WB; tested species: mouse

Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation.
Pechstein A, Tomilin N, Fredrich K, Vorontsova O, Sopova E, Evergren E, Haucke V, Brodin L, Shupliakov O
Cell reports (2020) 308: 2594-2602.e3. 106 011 WB

The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 106 011 WB, ICC; tested species: mouse

CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 106 011 WB; tested species: mouse

Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 106 011 WB; tested species: mouse

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 106 011 WB; tested species: mouse

Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions.
Ghézali G, Vasile F, Curry N, Fantham M, Cheung G, Ezan P, Cohen-Salmon M, Kaminski C, Rouach N
Cerebral cortex (New York, N.Y. : 1991) (2019) : . 106 011 WB; tested species: mouse

Connexin 30 controls astroglial polarization during postnatal brain development.
Ghézali G, Calvo CF, Pillet LE, Llense F, Ezan P, Pannasch U, Bemelmans AP, Etienne Manneville S, Rouach N
Development (Cambridge, England) (2018) 1454: . 106 011 WB; tested species: mouse

Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus.
Cicvaric A, Bulat T, Bormann D, Yang J, Auer B, Milenkovic I, Cabatic M, Milicevic R, Monje FJ
Food & function (2018) 93: 1532-1544. 106 011 WB; tested species: mouse

RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 106 011 WB; tested species: mouse

Synaptic control of mRNA translation by reversible assembly of XRN1 bodies.
Luchelli L, Thomas MG, Boccaccio GL
Journal of cell science (2015) 1288: 1542-54. 106 011 ICC, WB

Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.
Chowdhury D, Marco S, Brooks IM, Zandueta A, Rao Y, Haucke V, Wesseling JF, Tavalin SJ, Pérez-Otaño I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 4151-64. 106 011 WB

Working memory impairment in calcineurin knock-out mice is associated with alterations in synaptic vesicle cycling and disruption of high-frequency synaptic and network activity in prefrontal cortex.
Cottrell JR, Levenson JM, Kim SH, Gibson HE, Richardson KA, Sivula M, Li B, Ashford CJ, Heindl KA, Babcock RJ, Rose DM, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3327: 10938-49. 106 011 WB; tested species: mouse

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 106 011 WB; tested species: mouse

Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 106 011 WB

Bacterial cytolysin during meningitis disrupts the regulation of glutamate in the brain, leading to synaptic damage.
Wippel C, Maurer J, Förtsch C, Hupp S, Bohl A, Ma J, Mitchell TJ, Bunkowski S, Brück W, Nau R, Iliev AI, et al.
PLoS pathogens (2013) 96: e1003380. 106 011 IHC, WB; tested species: mouse

RIM proteins activate vesicle priming by reversing autoinhibitory homodimerization of Munc13.
Deng L, Kaeser PS, Xu W, Südhof TC
Neuron (2011) 692: 317-31. 106 011 ICC, WB; tested species: mouse

Suppression of guanylyl cyclase (beta1 subunit) expression impairs neurite outgrowth and synapse maturation in cultured cerebellar granule cells.
López-Jiménez ME, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
Cell death and differentiation (2009) 169: 1266-78. 106 011 ICC, WB; tested species: rat