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Home > Products > 111 111

SNAP25 antibody - 111 111

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Mouse monoclonal purified IgG
Cat. No.: 111 111
Amount: 50 µg
Price: $435.00
Cat. No. 111 111 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 1000 gallery  
ELISA: yes (see remarks)
Clone 71.2
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant rat SNAP25B protein (UniProt Id: P60881-1)
Epitop AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals, bovine.
No signal: zebrafish.
Other species not tested yet.
Specificity Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products.
Remarks

IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 111 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 111_111.pdf

References for SNAP25 - 111 111

Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.
Barker E, Milburn A, Helassa N, Hammond D, Sanchez-Soriano N, Morgan A, Barclay J
The Biochemical journal (2024) : . 111 111 WB; tested species: rat
Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
Pascual-Caro C, de Juan-Sanz J
PLoS biology (2024) 2210: e3002860. 111 111 WB; tested species: rat
LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Roppongi RT, Dhume SH, Padmanabhan N, Silwal P, Zahra N, Karimi B, Bomkamp C, Patil CS, Champagne-Jorgensen K, Twilley RE, Zhang P, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 111 111 WB; tested species: rat
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 111 111 WB; tested species: human
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.
Rathore SS, Liu Y, Yu H, Wan C, Lee M, Yin Q, Stowell MHB, Shen J
Cell reports (2019) 2913: 4583-4592.e3. 111 111 WB
Phosphorylated SNAP25 in the CA1 regulates morphine-associated contextual memory retrieval via increasing GluN2B-NMDAR surface localization.
Wang X, Liu Y, Jia M, Sun X, Wang N, Li Y, Cui C
Addiction biology (2017) : . 111 111 WB; tested species: rat
Identification and characterization of a novel botulinum neurotoxin.
Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Martínez-Carranza M, Dong M, Stenmark P
Nature communications (2017) 8: 14130. 111 111 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 111 111 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 111 111 WB; tested species: mouse
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 111 111 WB, IHC; tested species: mouse
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 111 111 WB; tested species: rat
Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 111 111 WB; tested species: human
Cat. No.: 111 111
Amount: 50 µg
Price: $435.00
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