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Home > Products > 111 002

SNAP25 antibody - 111 002

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Rabbit polyclonal antiserum
Cat. No.: 111 002
Amount: 200 µl
Price: $360.00
Cat. No. 111 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen Synthetic peptide corresponding to AA 192 to 206 from human SNAP25 (UniProt Id: P60880)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), hamster, chicken, goldfish, zebrafish.
Other species not tested yet.
Matching control protein/peptide 111-0P
Remarks

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 111 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 111_002.pdf

References for SNAP25 - 111 002

C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer's disease.
Yin X, Zhou H, Zhang M, Su J, Wang X, Li S, Yang Z, Kang Z, Zhou R
Nature communications (2023) 141: 5718. 111 002 WB, IHC; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 111 002 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 111 002 WB; tested species: mouse,human
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 111 002 WB; tested species: mouse
The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.
West ZE, Aitcheson SM, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2021) 2211: 368-376. 111 002 WB; tested species: mouse
miR-128 as a Regulator of Synaptic Properties in 5xFAD Mice Hippocampal Neurons.
Shvarts-Serebro I, Sheinin A, Gottfried I, Adler L, Schottlender N, Ashery U, Barak B
Journal of molecular neuroscience : MN (2021) : . 111 002 WB; tested species: mouse
SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.
Cardenas RA, Gonzalez R, Sanchez E, Ramos MA, Cardenas EI, Rodarte AI, Alcazar-Felix RJ, Isaza A, Burns AR, Heidelberger R, Adachi R, et al.
The Journal of biological chemistry (2021) 296: 100268. 111 002 WB; tested species: mouse
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 111 002 WB, IHC; tested species: mouse
ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 111 002 WB; tested species: mouse
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 002 WB; tested species: mouse
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 111 002 WB; tested species: mouse
SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 111 002 WB; tested species: monkey
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 111 002 WB; tested species: mouse
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 111 002 WB, ICC; tested species: mouse
Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 111 002 WB; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 111 002 WB; tested species: mouse,rat
Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 111 002 WB
Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 111 002 WB
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 002 WB; tested species: mouse
Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 111 002 WB, IHC
Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice.
Young FB, Franciosi S, Spreeuw A, Deng Y, Sanders S, Tam NC, Huang K, Singaraja RR, Zhang W, Bissada N, Kay C, et al.
PloS one (2012) 75: e36315. 111 002 WB; tested species: mouse
APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 111 002 WB; tested species: mouse
Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18.
Camoletto PG, Vara H, Morando L, Connell E, Marletto FP, Giustetto M, Sassoè-Pognetto M, Van Veldhoven PP, Ledesma MD
PloS one (2009) 44: e5310. 111 002 WB
An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function.
Johansson JU, Ericsson J, Janson J, Beraki S, Stanić D, Mandic SA, Wikström MA, Hökfelt T, Ogren SO, Rozell B, Berggren PO, et al.
PLoS genetics (2008) 411: e1000278. 111 002 WB, IHC; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 111 002 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 111 002 ICC; tested species: rat
Functional mapping of brain synapses by the enriching activity-marker SynaptoZip.
Ferro M, Lamanna J, Ripamonti M, Racchetti G, Arena A, Spadini S, Montesano G, Cortese R, Zimarino V, Malgaroli A
Nature communications (2017) 81: 1229. 111 002 ICC; tested species: rat
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 111 002 WB, ICC; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis.
Fossati G, Morini R, Corradini I, Antonucci F, Trepte P, Edry E, Sharma V, Papale A, Pozzi D, Defilippi P, Meier JC, et al.
Cell death and differentiation (2015) 229: 1425-36. 111 002 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 111 002 ICC
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 111 002 ICC; tested species: mouse
C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer's disease.
Yin X, Zhou H, Zhang M, Su J, Wang X, Li S, Yang Z, Kang Z, Zhou R
Nature communications (2023) 141: 5718. 111 002 WB, IHC; tested species: mouse
Activity-dependent somatodendritic dopamine release in the substantia nigra autoinhibits the releasing neuron.
Hikima T, Lee CR, Witkovsky P, Chesler J, Ichtchenko K, Rice ME
Cell reports (2021) 351: 108951. 111 002 IHC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 111 002 IHC; tested species: human
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 111 002 WB, IHC; tested species: mouse
Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells.
Zhang E, Mohammed Al-Amily I, Mohammed S, Luan C, Asplund O, Ahmed M, Ye Y, Ben-Hail D, Soni A, Vishnu N, Bompada P, et al.
Cell metabolism (2018) : . 111 002 IHC; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 002 IHC; tested species: mouse
Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 111 002 IHC
Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 111 002 WB, IHC
An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function.
Johansson JU, Ericsson J, Janson J, Beraki S, Stanić D, Mandic SA, Wikström MA, Hökfelt T, Ogren SO, Rozell B, Berggren PO, et al.
PLoS genetics (2008) 411: e1000278. 111 002 WB, IHC; tested species: mouse
Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 111 002 IHC; tested species: human
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 111 002 ELISA
Cat. No.: 111 002
Amount: 200 µl
Price: $360.00
C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer's disease.
Yin X, Zhou H, Zhang M, Su J, Wang X, Li S, Yang Z, Kang Z, Zhou R
Nature communications (2023) 141: 5718. 111 002 WB, IHC; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 111 002 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 111 002 WB; tested species: mouse,human
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 111 002 WB; tested species: mouse
The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.
West ZE, Aitcheson SM, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2021) 2211: 368-376. 111 002 WB; tested species: mouse
miR-128 as a Regulator of Synaptic Properties in 5xFAD Mice Hippocampal Neurons.
Shvarts-Serebro I, Sheinin A, Gottfried I, Adler L, Schottlender N, Ashery U, Barak B
Journal of molecular neuroscience : MN (2021) : . 111 002 WB; tested species: mouse
SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.
Cardenas RA, Gonzalez R, Sanchez E, Ramos MA, Cardenas EI, Rodarte AI, Alcazar-Felix RJ, Isaza A, Burns AR, Heidelberger R, Adachi R, et al.
The Journal of biological chemistry (2021) 296: 100268. 111 002 WB; tested species: mouse
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 111 002 WB, IHC; tested species: mouse
ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 111 002 WB; tested species: mouse
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 002 WB; tested species: mouse
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 111 002 WB; tested species: mouse
SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 111 002 WB; tested species: monkey
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 111 002 WB; tested species: mouse
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 111 002 WB, ICC; tested species: mouse
Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 111 002 WB; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 111 002 WB; tested species: mouse,rat
Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 111 002 WB
Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 111 002 WB
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 002 WB; tested species: mouse
Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 111 002 WB, IHC
Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice.
Young FB, Franciosi S, Spreeuw A, Deng Y, Sanders S, Tam NC, Huang K, Singaraja RR, Zhang W, Bissada N, Kay C, et al.
PloS one (2012) 75: e36315. 111 002 WB; tested species: mouse
APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 111 002 WB; tested species: mouse
Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18.
Camoletto PG, Vara H, Morando L, Connell E, Marletto FP, Giustetto M, Sassoè-Pognetto M, Van Veldhoven PP, Ledesma MD
PloS one (2009) 44: e5310. 111 002 WB
An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function.
Johansson JU, Ericsson J, Janson J, Beraki S, Stanić D, Mandic SA, Wikström MA, Hökfelt T, Ogren SO, Rozell B, Berggren PO, et al.
PLoS genetics (2008) 411: e1000278. 111 002 WB, IHC; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 111 002 ICC; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 111 002 ICC; tested species: rat
Functional mapping of brain synapses by the enriching activity-marker SynaptoZip.
Ferro M, Lamanna J, Ripamonti M, Racchetti G, Arena A, Spadini S, Montesano G, Cortese R, Zimarino V, Malgaroli A
Nature communications (2017) 81: 1229. 111 002 ICC; tested species: rat
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 111 002 WB, ICC; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis.
Fossati G, Morini R, Corradini I, Antonucci F, Trepte P, Edry E, Sharma V, Papale A, Pozzi D, Defilippi P, Meier JC, et al.
Cell death and differentiation (2015) 229: 1425-36. 111 002 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 111 002 ICC
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 111 002 ICC; tested species: mouse
C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer's disease.
Yin X, Zhou H, Zhang M, Su J, Wang X, Li S, Yang Z, Kang Z, Zhou R
Nature communications (2023) 141: 5718. 111 002 WB, IHC; tested species: mouse
Activity-dependent somatodendritic dopamine release in the substantia nigra autoinhibits the releasing neuron.
Hikima T, Lee CR, Witkovsky P, Chesler J, Ichtchenko K, Rice ME
Cell reports (2021) 351: 108951. 111 002 IHC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 111 002 IHC; tested species: human
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 111 002 WB, IHC; tested species: mouse
Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells.
Zhang E, Mohammed Al-Amily I, Mohammed S, Luan C, Asplund O, Ahmed M, Ye Y, Ben-Hail D, Soni A, Vishnu N, Bompada P, et al.
Cell metabolism (2018) : . 111 002 IHC; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 002 IHC; tested species: mouse
Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 111 002 IHC
Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 111 002 WB, IHC
An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function.
Johansson JU, Ericsson J, Janson J, Beraki S, Stanić D, Mandic SA, Wikström MA, Hökfelt T, Ogren SO, Rozell B, Berggren PO, et al.
PLoS genetics (2008) 411: e1000278. 111 002 WB, IHC; tested species: mouse
Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 111 002 IHC; tested species: human
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 111 002 ELISA
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Background

SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.
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