Tailor-made Antibodies
and Tools for Life Science

SNAP25 antibody - 111 002

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Rabbit polyclonal antiserum
Cat. No.: 111 002
Amount: 200 µl
Price: $365.00
Cat. No. 111 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 192 to 206 from human SNAP25 (UniProt Id: P60880)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), hamster, chicken, goldfish, zebrafish.
Other species not tested yet.
Matching control protein/peptide 111-0P
Remarks

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 111 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 111_002.pdf

References for SNAP25 - 111 002

Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 111 002 WB; tested species: mouse
C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer's disease.
Yin X, Zhou H, Zhang M, Su J, Wang X, Li S, Yang Z, Kang Z, Zhou R
Nature communications (2023) 141: 5718. 111 002 WB, IHC; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 111 002 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 111 002 WB; tested species: mouse,human
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 111 002 WB; tested species: mouse
SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.
Cardenas RA, Gonzalez R, Sanchez E, Ramos MA, Cardenas EI, Rodarte AI, Alcazar-Felix RJ, Isaza A, Burns AR, Heidelberger R, Adachi R, et al.
The Journal of biological chemistry (2021) 296: 100268. 111 002 WB; tested species: mouse
The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.
West ZE, Aitcheson SM, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2021) 2211: 368-376. 111 002 WB; tested species: mouse
miR-128 as a Regulator of Synaptic Properties in 5xFAD Mice Hippocampal Neurons.
Shvarts-Serebro I, Sheinin A, Gottfried I, Adler L, Schottlender N, Ashery U, Barak B
Journal of molecular neuroscience : MN (2021) : . 111 002 WB; tested species: mouse
ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 111 002 WB; tested species: mouse
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 111 002 WB, IHC; tested species: mouse
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 002 WB; tested species: mouse
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 111 002 WB; tested species: mouse
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 111 002 WB; tested species: mouse
SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 111 002 WB; tested species: monkey
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 111 002 WB; tested species: mouse,rat
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 002 WB, IP, ICC; tested species: rat
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 111 002 WB, ICC; tested species: mouse
Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 111 002 WB; tested species: mouse
Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology.
Bodaleo FJ, Montenegro-Venegas C, Henríquez DR, Court FA, Gonzalez-Billault C
Scientific reports (2016) 6: 30069. 111 002 WB
Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 111 002 WB
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 002 WB; tested species: mouse
Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 111 002 WB, IHC
Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice.
Young FB, Franciosi S, Spreeuw A, Deng Y, Sanders S, Tam NC, Huang K, Singaraja RR, Zhang W, Bissada N, Kay C, et al.
PloS one (2012) 75: e36315. 111 002 WB; tested species: mouse
APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 111 002 WB; tested species: mouse
Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18.
Camoletto PG, Vara H, Morando L, Connell E, Marletto FP, Giustetto M, Sassoè-Pognetto M, Van Veldhoven PP, Ledesma MD
PloS one (2009) 44: e5310. 111 002 WB
An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function.
Johansson JU, Ericsson J, Janson J, Beraki S, Stanić D, Mandic SA, Wikström MA, Hökfelt T, Ogren SO, Rozell B, Berggren PO, et al.
PLoS genetics (2008) 411: e1000278. 111 002 WB, IHC; tested species: mouse
Cat. No.: 111 002
Amount: 200 µl
Price: $365.00