AP180 peptide - 155-0P

AP180 is a synaptic protein involved in clathrin mediated endocytosis

control peptide

Cat. No.: 155-0P

Amount: 100 µg

Price: $105.00

Cat. No. 155-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	155 002, 155 003
Remarks	This control peptide consists of the synthetic peptide that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	155-0p.pdf

Cat. No.: 155-0P

Amount: 100 µg

Price: $105.00

Background

During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of e.g. amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
AP 180 also known as pp155, NP185, F1-20, and SNAP 91 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats.

1 ) Bao H et al., J. Neurophysiol. (2005)
2 ) Yao PJ et al., J. Comp. Neurol. (2005)
3 ) Yao PJ et al., J. Comp. Neurol. (2002)
4 ) Kalthoff C et al., J. Biol. Chem. (2002)
5 ) Yao PJ et al., Neuroscience (1999)
6 ) Hao W et al., J. Biol. Chem. (1999)
7 ) Morris SA et al., EMBO J. (1993)
8 ) Prasad K et al., Biochemistry (1988)

Protocols

OTHER

Preadsorbtion

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