Synaptic Systems

Cat. No.: 155 003

Amount: 50 µg

Price: $460.00

Cat. No. 155 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 1000 gallery IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Reactivity	Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog. Other species not tested yet.
Specificity	Specific for AP 180. K.O. validated PubMed: 26412491
Matching control protein/peptide	155-0P
Data sheet	155_003.pdf

Physical exercise mediates cortical synaptic protein lactylation to improve stress resilience.
Yan L, Wang Y, Hu H, Yang D, Wang W, Luo Z, Wang Y, Yang F, So KF, Zhang L
Cell metabolism (2024) 369: 2104-2117.e4. 155 003 WB, IP, IHC; tested species: mouse

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 155 003 WB; KO verified; tested species: mouse

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse

Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 155 003 WB; KO verified; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat

Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 155 003 WB

Cat. No.: 155 003

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

Protocols

AP180 antibody - 155 003 K.O.