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AP 180 - 155 003

A synaptic protein involved in clathrin mediated endocytosis
Polyclonal rabbit purified antibody
Cat. No.: 155 003
Amount: 50 µg
Price: $450.00
Cat. No. 155 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 1000 gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Reactivity Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog.
Other species not tested yet.
Specificity Specific for AP 180. K.O. PubMed: 26412491
Matching control protein/peptide 155-0P
Data sheet 155_003.pdf

References for AP 180 - 155 003

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 155 003 WB; KO verified; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 155 003 WB
Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.
Gimber N, Tadeus G, Maritzen T, Schmoranzer J, Haucke V
Nature communications (2015) 6: 8392. 155 003 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition.
Geis C, Weishaupt A, Hallermann S, Grünewald B, Wessig C, Wultsch T, Reif A, Byts N, Beck M, Jablonka S, Boettger MK, et al.
Brain : a journal of neurology (2010) 13311: 3166-80. 155 003 ICC
Quantitative Fluorescent in situ Hybridization Reveals Differential Transcription Profile Sharpening of Endocytic Proteins in Cochlear Hair Cells Upon Maturation.
Huang G, Eckrich S
Frontiers in cellular neuroscience (2021) 15: 643517. 155 003 IHC; tested species: mouse
AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells.
Kroll J, Özçete ÖD, Jung S, Maritzen T, Milosevic I, Wichmann C, Moser T
Journal of cell science (2020) 1332: . 155 003 IHC; KO verified; tested species: mouse
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 155 003 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Cat. No.: 155 003
Quantity: 50 µg
Price: $450.00
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 155 003 WB; KO verified; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 155 003 WB
Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.
Gimber N, Tadeus G, Maritzen T, Schmoranzer J, Haucke V
Nature communications (2015) 6: 8392. 155 003 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition.
Geis C, Weishaupt A, Hallermann S, Grünewald B, Wessig C, Wultsch T, Reif A, Byts N, Beck M, Jablonka S, Boettger MK, et al.
Brain : a journal of neurology (2010) 13311: 3166-80. 155 003 ICC
Quantitative Fluorescent in situ Hybridization Reveals Differential Transcription Profile Sharpening of Endocytic Proteins in Cochlear Hair Cells Upon Maturation.
Huang G, Eckrich S
Frontiers in cellular neuroscience (2021) 15: 643517. 155 003 IHC; tested species: mouse
AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells.
Kroll J, Özçete ÖD, Jung S, Maritzen T, Milosevic I, Wichmann C, Moser T
Journal of cell science (2020) 1332: . 155 003 IHC; KO verified; tested species: mouse
Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 155 003 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat
Background

During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of e.g. amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
AP 180 also known as pp155, NP185, F1-20, and SNAP 91 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats.