Synaptic Systems

Cat. No.: 155 002

Amount: 200 µl

Price: $355.00

Cat. No. 155 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not recommended (see remarks) IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Reactivity	Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog. Other species not tested yet.
Specificity	Specific for AP 180.
Matching control protein/peptide	155-0P
Remarks	ICC: The affinity-purified antibody (cat. no. 155 003) is recommended.
Data sheet	155_002.pdf

References for AP180 - 155 002

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 155 002 WB; tested species: mouse

Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 155 002 WB; tested species: mouse

Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.
Farsi Z, Gowrisankaran S, Krunic M, Rammner B, Woehler A, Lafer EM, Mim C, Jahn R, Milosevic I
eLife (2018) 7: . 155 002 WB; tested species: mouse

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 155 002 WB; tested species: mouse

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 155 002

Cat. No.: 155 002

Amount: 200 µl

Price: $355.00

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 155 002 WB; tested species: mouse

Background

During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of e.g. amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
AP 180 also known as pp155, NP185, F1-20, and SNAP 91 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats.

1 ) Bao H et al., J. Neurophysiol. (2005)
2 ) Yao PJ et al., J. Comp. Neurol. (2005)
3 ) Yao PJ et al., J. Comp. Neurol. (2002)
4 ) Kalthoff C et al., J. Biol. Chem. (2002)
5 ) Yao PJ et al., Neuroscience (1999)
6 ) Hao W et al., J. Biol. Chem. (1999)
7 ) Morris SA et al., EMBO J. (1993)
8 ) Prasad K et al., Biochemistry (1988)

Protocols

OTHER

Preabsorbtion

Other products for this target

AP180 antibody - 155 002

References for AP180 - 155 002