Cat. No.: 123 011
Amount: 100 µg
Price:
$415.00
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| Cat. No. 123 011 |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 83.11 |
| Subtype | IgG1 |
| Immunogen | Recombinant protein corresponding to AA 1 to 744 from rat NSF (UniProt Id: Q9QUL6) |
| Reactivity |
Reacts with: rat (Q9QUL6), mouse (P46460). No signal: zebrafish. Other species not tested yet. |
| Remarks |
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 123 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended. |
| Data sheet | 123_011.pdf |
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N-ethylamide sensitive fusion protein NSF functions together with SNAPs (soluble NSF attachment proteins) and SNAREs (SNAP receptors) in vesicular transport.
NSF is a homotrimer whose polypeptide subunits are made up of three distinct domains: an amino - terminal domain (N) and two homologous ATP-binding domains (D1 and D2). NSF is an ATPase that dissociates SNARE complexes, such as the core complex composed of synaptobrevin/VAMP, syntaxin 1 and SNAP 25 under ATP hydrolysis. The ability of the D1 domain to hydrolyze ATP is required for NSF activity. The D2 domain is required for trimerization, but its ability to hydrolyze ATP is not absolutely required for NSF function.