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Home > Products > 123 002

NSF antibody - 123 002

NSF is a trimeric ATPase required for membrane fusion during vesicular transport
Rabbit polyclonal antiserum
Cat. No.: 123 002
Amount: 200 µl
Price: $360.00
Cat. No. 123 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: yes
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 733 to 744 from rat NSF (UniProt Id: Q9QUL6)
Reactivity Reacts with: human (P46459), rat (Q9QUL6), mouse (P46460), hamster.
Other species not tested yet.
Matching control protein/peptide 123-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 123 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 123_002.pdf

References for NSF - 123 002

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 123 002 WB; tested species: mouse
A connection between reversible tyrosine phosphorylation and SNARE complex-disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E.
Ruete MC, Zarelli VEP, Masone D, Paola M, Bustos DM, Tomes CN
Molecular human reproduction (2019) : . 123 002 WB; tested species: human
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 123 002 WB; tested species: mouse
Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 123 002 WB
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells.
Tian JH, Wu ZX, Unzicker M, Lu L, Cai Q, Li C, Schirra C, Matti U, Stevens D, Deng C, Rettig J, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10546-55. 123 002 WB
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Michaut M, Tomes CN, De Blas G, Yunes R, Mayorga LS
Proceedings of the National Academy of Sciences of the United States of America (2000) 9718: 9996-10001. 123 002 WB, ICC
Cat. No.: 123 002
Amount: 200 µl
Price: $360.00
WB
ICC
IHC
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