Synaptic Systems - Synaptogyrin1

Cat. No.: 103 011

Amount: 50 µg

Price: $415.00

Cat. No. 103 011	50 µg purified IgG, lyophilized. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not recommended IHC: not recommended IHC-P: not recommended ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	80.1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 170 to 234 from rat Synaptogyrin1 (UniProt Id: Q62876)
Reactivity	Reacts with: human (O43759), rat (Q62876), mouse (O55100), hamster. No signal: zebrafish. Other species not tested yet.
Specificity	Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.
Remarks	IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 103 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	103_011.pdf

References for Synaptogyrin1 - 103 011

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 103 011 WB

Structure of synaptogyrin (p29) defines novel synaptic vesicle protein.
Stenius K, Janz R, Südhof TC, Jahn R
The Journal of cell biology (1995) 1316 Pt 2: 1801-9. 103 011 WB, ICC

The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 103 011 IHC

Distinctive alteration of presynaptic proteins in the outer molecular layer of the dentate gyrus in Alzheimer's disease.
Haytural H, Jordà-Siquier T, Winblad B, Mulle C, Tjernberg LO, Granholm AC, Frykman S, Barthet G
Brain communications (2021) 32: fcab079. 103 011 IHC-P; tested species: human

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 103 011

Cat. No.: 103 011

Amount: 50 µg

Price: $415.00

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 103 011 WB

Structure of synaptogyrin (p29) defines novel synaptic vesicle protein.
Stenius K, Janz R, Südhof TC, Jahn R
The Journal of cell biology (1995) 1316 Pt 2: 1801-9. 103 011 WB, ICC

Background

Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.

1 ) Janz R et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Janz R et al., J. Biol. Chem. (1998)
4 ) Südhof TC et al., Nature (1995)

Protocols

Standard protocol

ELISA

Membrane proteins

Other products for this target

Synaptogyrin1 antibody - 103 011

References for Synaptogyrin1 - 103 011