Tailor-made Antibodies
and Tools for Life Science

Synaptogyrin1 antibody - 103 011

Synaptogyrin is a major synaptic vesicle membrane protein; tyrosine-phosphorylated
Mouse monoclonal purified IgG
Cat. No.: 103 011
Amount: 50 µg
Price: $420.00
Cat. No. 103 011 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not recommended
IHC: not recommended
IHC-P: not recommended
ELISA: yes (see remarks)
Clone 80.1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 170 to 234 from rat Synaptogyrin1 (UniProt Id: Q62876)
Reactivity Reacts with: human (O43759), rat (Q62876), mouse (O55100), hamster.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.
Remarks

IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 103 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 103_011.pdf

References for Synaptogyrin1 - 103 011

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 103 011 WB
Structure of synaptogyrin (p29) defines novel synaptic vesicle protein.
Stenius K, Janz R, Südhof TC, Jahn R
The Journal of cell biology (1995) 1316 Pt 2: 1801-9. 103 011 WB, ICC
Cat. No.: 103 011
Amount: 50 µg
Price: $420.00