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SYSY - Protocols for Various Applications

Western blot (WB) protocols
In Western blot (WB) assays, protein samples are separated according to their molecular weight with denaturing SDS-PAGE, transferred to a membrane and analyzed by immuno-detection with antibodies. Different detection methods like enhanced chemiluminescence (ECL), alkaline phosphatase staining (AP) and fluorescent detection are available.

Immunocytochemistry (ICC) protocols
In ICC applications, fixed or unfixed cells are immunostained with antibodies. In general fluorescent detection systems are employed. This allows the analysis of the subcellular localization of several proteins at once.

Immunohistochemistry (IHC) protocols
In IHC applications, tissue samples are immunostained with antibodies. In general fluorescent or chromogenic detection systems are employed. This allows the analysis of tissue specific protein expression patterns on the cellular and subcellular level. 

Immunohistochemistry paraffin (IHC-P) protocols
Formalin-fixed paraffin-embedded (FFPE) tissues are widely used in clinical and pre-clinical studies. Preparation of the tissue samples is critical for subsequent fluorescent or chromogenic immunohistochemial analysis.  

Immunoprecipitation (IP) protocols
In IP experiments, target molecules and potential interaction partners are isolated from complex cell or tissue lysates by specific binding to capture antibodies.

Enzyme-linked Immunosorbent Assay (ELISA) protocols
In ELISA approaches, target molecules (proteins, antibodies, hormones or other low molecular weight compounds) are immunologically detected and quantified by antibodies. Different detection methods (colormetric, fluorescent, radioactive etc.) are availble.

Other protocols
Find here some special SYSY protocols for the preparation of tissue fractions and antibody purification.