Tailor-made Antibodies
and Tools for Life Science

VGAT antibody cytoplasmic domain - 131 011 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 131 011
Amount: 100 µg
Price: $440.00
Cat. No. 131 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 1000 gallery  
IHC: 1 : 100 up to 1 : 1000 gallery  
IHC-P: 1 : 100 up to 1 : 500 gallery  
ExM: yes (see remarks) gallery  
DNA-PAINT: yes
Clarity: 1 : 50 (see remarks)
EM: yes
FACS: yes
Clone 117G4
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, monkey.
Other species not tested yet.
Specificity K.O. validated PubMed: 29431653
Matching control protein/peptide 131-0P
Remarks

WB:  VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453).
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).

Data sheet 131_011.pdf

References for VGAT - 131 011

The impact of exogenous Oxytocin on visual cortex plasticity across different stages of visual development.
Sun Y, Wang X, Chen Y, Luan Z, Hao R
Scientific reports (2025) 151: 12137. 131 011 WB, IHC; tested species: mouse
The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 131 011 WB, ICC; tested species: mouse
Microglial lipid phosphatase SHIP1 limits complement-mediated synaptic pruning in the healthy developing hippocampus.
Matera A, Compagnion AC, Pedicone C, Janssen MK, Ivanov A, Monsorno K, Labouèbe G, Leggio L, Pereira M, Beule D, Mansuy-Aubert V, et al.
Immunity (2024) : . 131 011 WB; tested species: mouse
GABAergic/Glycinergic and Glutamatergic Neurons Mediate Distinct Neurodevelopmental Phenotypes of STXBP1 Encephalopathy.
Kim JH, Chen W, Chao ES, Rivera A, Kaku HN, Jiang K, Lee D, Chen H, Vega JM, Chin TV, Jin K, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4414: . 131 011 WB; tested species: mouse
Impairments of GABAergic transmission in hippocampus mediate increased susceptibility of epilepsy in the early stage of Alzheimer's disease.
Mao R, Hu M, Liu X, Ye L, Xu B, Sun M, Xu S, Shao W, Tan Y, Xu Y, Bai F, et al.
Cell communication and signaling : CCS (2024) 221: 147. 131 011 WB; tested species: mouse
LPS induces microglial activation and GABAergic synaptic deficits in the hippocampus accompanied by prolonged cognitive impairment.
Jung H, Lee D, You H, Lee M, Kim H, Cheong E, Um JW
Scientific reports (2023) 131: 6547. 131 011 WB, IHC; tested species: mouse
Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 131 011 WB; tested species: mouse
Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction.
Ruggiero-Ruff RE, Villa PA, Hijleh SA, Avalos B, DiPatrizio NV, Haga-Yamanaka S, Coss D
Scientific reports (2023) 131: 12666. 131 011 WB, IHC; tested species: mouse
Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 131 011 WB; tested species: mouse
Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons.
Ruiz-Reig N, García-Sánchez D, Schakman O, Gailly P, Tissir F
Frontiers in molecular neuroscience (2022) 15: 1110986. 131 011 WB, IHC; tested species: mouse
Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 131 011 WB; tested species: mouse
Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 131 011 WB; tested species: mouse
Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor function.
Kawai M, Imaizumi K, Ishikawa M, Shibata S, Shinozaki M, Shibata T, Hashimoto S, Kitagawa T, Ago K, Kajikawa K, Shibata R, et al.
Cell reports (2021) 378: 110019. 131 011 WB, ICC; tested species: mouse
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 131 011 WB, ICC; tested species: mouse
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 131 011 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 131 011 WB; tested species: rat
Characterization of kindled VGAT-Cre mice as a new animal model of temporal lobe epilepsy.
Straub J, Gawda A, Ravichandran P, McGrew B, Nylund E, Kang J, Burke C, Vitko I, Scott M, Williamson J, Joshi S, et al.
Epilepsia (2020) : . 131 011 WB, IHC; tested species: mouse
A complement-microglial axis driving inhibitory synapse related protein loss might contribute to systemic inflammation-induced cognitive impairment.
Li SM, Li B, Zhang L, Zhang GF, Sun J, Ji MH, Yang JJ
International immunopharmacology (2020) 87: 106814. 131 011 WB, IHC; tested species: mouse
Targeting PPARα in the rat valproic acid model of autism: focus on social motivational impairment and sex-related differences.
Scheggi S, Guzzi F, Braccagni G, De Montis MG, Parenti M, Gambarana C
Molecular autism (2020) 111: 62. 131 011 WB; tested species: rat
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 131 011 WB; tested species: mouse
A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 131 011 WB, IHC; tested species: mouse
Mechanisms of hyperexcitability in Alzheimer's disease hiPSC-derived neurons and cerebral organoids vs. isogenic control.
Ghatak S, Dolatabadi N, Trudler D, Zhang X, Wu Y, Mohata M, Ambasudhan R, Talantova M, Lipton SA
eLife (2019) 8: . 131 011 WB, ICC; tested species: human
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 131 011 WB, ICC, IHC; tested species: rat
Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
Chmielewska JJ, Kuzniewska B, Milek J, Urbanska K, Dziembowska M
Molecular neurobiology (2018) : . 131 011 WB; tested species: mouse
Gene Expression Profile in Frontal Cortex in Sporadic Frontotemporal Lobar Degeneration-TDP.
Andrés-Benito P, Gelpi E, Povedano M, Santpere G, Ferrer I
Journal of neuropathology and experimental neurology (2018) : . 131 011 WB; tested species: human
Altered sensory processing and dendritic remodeling in hyperexcitable visual cortical networks.
Vannini E, Restani L, Pietrasanta M, Panarese A, Mazzoni A, Rossetto O, Middei S, Micera S, Caleo M
Brain structure & function (2016) 2216: 2919-36. 131 011 WB
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 131 011 IHC, WB
EphA4 is localized in clathrin-coated and synaptic vesicles in adult mouse brain.
Bouvier D, Tremblay ME, Riad M, Corera AT, Gingras D, Horn KE, Fotouhi M, Girard M, Murai KK, Kennedy TE, McPherson PS, et al.
Journal of neurochemistry (2010) 1131: 153-65. 131 011 ICC, IP, WB
Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons.
Tafoya LC, Mameli M, Miyashita T, Guzowski JF, Valenzuela CF, Wilson MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2630: 7826-38. 131 011 WB, ICC, IHC
Cat. No.: 131 011
Amount: 100 µg
Price: $440.00