Synaptic Systems - alpha Synuclein

Cat. No.: 128 211

Amount: 100 µg

Price: $425.00

Cat. No. 128 211	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery DNA-PAINT: yes (see remarks) ELISA: yes (see remarks)
Clone	354A10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of human α-Synuclein. (UniProt Id: P37840)
Reactivity	Reacts with: human (P37840), rat (P37377), mouse (O55042), mammals. Other species not tested yet.
Specificity	Specific for α-synuclein, no cross-reactivity to β- and γ-synuclein.
Remarks	DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614). ELISA: This antibody is suitable as capture antibody for sandwich-ELISA with cat. no. 128 003 as detector antibody.
Data sheet	128_211.pdf

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 128 211 WB, IHC; tested species: mouse

N-terminal acetylation mutants affect alpha-synuclein stability, protein levels and neuronal toxicity.
Vinueza-Gavilanes R, Íñigo-Marco I, Larrea L, Lasa M, Carte B, Santamaría E, Fernández-Irigoyen J, Bugallo R, Aragón T, Aldabe R, Arrasate M, et al.
Neurobiology of disease (2020) : 104781. 128 211 WB, ICC; tested species: human

Cat. No.: 128 211

Amount: 100 µg

Price: $425.00

ICC

IHC

IHC-P

Background

Protocols

alpha Synuclein antibody - 128 211