Cat. No.: 110-1P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 110-1P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to cytoplasmic-domain without membrane anchor of rat Syntaxin 1A. (UniProt Id: P32851) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 110 011, 110 011C3, 110 111, 110 118, 110 318 |
| Remarks |
This control protein consists of aa 1 - 262 of rat syntaxin 1A in 2M Urea / TBS. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 110-1p.pdf |
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Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca2+-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.