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Home > Products > 110 111

Syntaxin1A antibody - 110 111 K.O.

Syntaxin1 is a plasma membrane SNARE protein essential for synaptic vesicle fusion
Mouse monoclonal purified IgG
Cat. No.: 110 111
Amount: 100 µg
Price: $415.00
Cat. No. 110 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 200 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: yes
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone 78.3
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to cytoplasmic-domain without membrane anchor of rat Syntaxin 1A. (UniProt Id: P32851)
Reactivity Reacts with: human (Q16623), rat (P32851), mouse (O35526), mammals, chicken.
Other species not tested yet.
Specificity Specific for syntaxin 1A, no cross-reactivity to syntaxin 1B. K.O. validated PubMed: 38512129
Matching control protein/peptide 110-1P
Remarks

IP: Immunoprecipitates syntaxin1A including complexes with synaptobrevin and SNAP25.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet 110_111.pdf

References for Syntaxin1A - 110 111

Hippocampal excitation-inhibition balance underlies the 5-HT2C receptor in modulating depressive behaviours.
Shi HJ, Xue YR, Shao H, Wei C, Liu T, He J, Yang YH, Wang HM, Li N, Ren SQ, Chang L, et al.
Brain : a journal of neurology (2024) : . 110 111 WB; tested species: mouse
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 111 WB, ICC; KO verified; tested species: mouse
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 110 111 WB; tested species: human
The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 110 111 WB; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 110 111 WB, IP; tested species: mouse
SNAP23 regulates KCC2 membrane insertion and activity following mZnR/GPR39 activation in hippocampalneurons.
Asraf H, Bogdanovic M, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
iScience (2022) 252: 103751. 110 111 WB; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 110 111 WB, IHC; tested species: mouse
Hyperglycemia-Induced Dysregulated Fusion Intermediates in Insulin-Secreting Cells Visualized by Super-Resolution Microscopy.
Yang G, Li L, Liu Y, Liang K, Wei L, Chen L
Frontiers in cell and developmental biology (2021) 9: 650167. 110 111 WB; tested species: rat
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 110 111 WB; tested species: mouse
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 110 111 WB; KD verified; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 110 111 WB; tested species: mouse
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 110 111 WB; tested species: human
Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes.
Duan MJ, Yan ML, Wang Q, Mao M, Su D, Sun LL, Li KX, Qu Y, Sun Q, Zhang XY, Huang SY, et al.
Cell communication and signaling : CCS (2018) 161: 91. 110 111 WB; tested species: mouse
CRISPR interference-based specific and efficient gene inactivation in the brain.
Zheng Y, Shen W, Zhang J, Yang B, Liu YN, Qi H, Yu X, Lu SY, Chen Y, Xu YZ, Li Y, et al.
Nature neuroscience (2018) 213: 447-454. 110 111 WB; KD verified; tested species: mouse
Analysis of SUMO1-conjugation at synapses.
Daniel JA, Cooper BH, Palvimo JJ, Zhang FP, Brose N, Tirard M
eLife (2017) 6: . 110 111 WB; tested species: mouse
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 110 111 WB
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 110 111 WB; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 111 WB, EM
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 110 111 WB; tested species: mouse
Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus.
Araya-Callís C, Hiemke C, Abumaria N, Flugge G
Psychopharmacology (2012) 2241: 209-22. 110 111 WB; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 110 111 WB
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 110 111 WB, ICC
SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions.
Lam AD, Tryoen-Toth P, Tsai B, Vitale N, Stuenkel EL
Molecular biology of the cell (2008) 192: 485-97. 110 111 WB, IP; tested species: rat
Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
Journal of cell science (2004) 117Pt 10: 1923-35. 110 111 WB, ICC
The interaction between syntaxin 1A and cystic fibrosis transmembrane conductance regulator Cl- channels is mechanistically distinct from syntaxin 1A-SNARE interactions.
Ganeshan R, Di A, Nelson DJ, Quick MW, Kirk KL
The Journal of biological chemistry (2003) 2785: 2876-85. 110 111 WB
The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.
Hatsuzawa K, Lang T, Fasshauer D, Bruns D, Jahn R
The Journal of biological chemistry (2003) 27833: 31159-66. 110 111 WB, ICC
Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.
Varoqueaux F, Sigler A, Rhee JS, Brose N, Enk C, Reim K, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2002) 9913: 9037-42. 110 111 WB
Regulation of Neural Circuit Development by Cadherin-11 Provides Implications for Autism.
Frei JA, Niescier RF, Bridi MS, Durens M, Nestor JE, Kilander MBC, Yuan X, Dykxhoorn DM, Nestor MW, Huang S, Blatt GJ, et al.
eNeuro () 84: . 110 111 WB; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 110 111 WB, IP; tested species: mouse
SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions.
Lam AD, Tryoen-Toth P, Tsai B, Vitale N, Stuenkel EL
Molecular biology of the cell (2008) 192: 485-97. 110 111 WB, IP; tested species: rat
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 111 WB, ICC; KO verified; tested species: mouse
Altered Distribution of SNARE Proteins in Primary Neurons Exposed to Different Alpha-Synuclein Proteoforms.
Brolin E, Ingelsson M, Bergström J, Erlandsson A
Cellular and molecular neurobiology (2023) : . 110 111 ICC; tested species: mouse
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 110 111 ICC; tested species: rat
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 111 ICC; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 110 111 WB, ICC
Syntaxin1A lateral diffusion reveals transient and local SNARE interactions.
Ribrault C, Reingruber J, Petković M, Galli T, Ziv NE, Holcman D, Triller A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3148: 17590-602. 110 111 ICC; tested species: rat
A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.
Jiang X, Litkowski PE, Taylor AA, Lin Y, Snider BJ, Moulder KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 305: 1798-809. 110 111 ICC
A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.
Barszczewski M, Chua JJ, Stein A, Winter U, Heintzmann R, Zilly FE, Fasshauer D, Lang T, Jahn R
Molecular biology of the cell (2008) 193: 776-84. 110 111 ICC
Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
Journal of cell science (2004) 117Pt 10: 1923-35. 110 111 WB, ICC
The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.
Hatsuzawa K, Lang T, Fasshauer D, Bruns D, Jahn R
The Journal of biological chemistry (2003) 27833: 31159-66. 110 111 WB, ICC
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 110 111 WB, IHC; tested species: mouse
Lipopolysaccharide augments microglial GABA uptake by increasing GABA transporter-1 trafficking and bestrophin-1 expression.
Di Palma M, Catalano M, Serpe C, De Luca M, Monaco L, Kunzelmann K, Limatola C, Conti F, Fattorini G
Glia (2023) 7111: 2527-2540. 110 111 DNA-PAINT; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 111 WB, EM
Cat. No.: 110 111
Amount: 100 µg
Price: $415.00
Hippocampal excitation-inhibition balance underlies the 5-HT2C receptor in modulating depressive behaviours.
Shi HJ, Xue YR, Shao H, Wei C, Liu T, He J, Yang YH, Wang HM, Li N, Ren SQ, Chang L, et al.
Brain : a journal of neurology (2024) : . 110 111 WB; tested species: mouse
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 111 WB, ICC; KO verified; tested species: mouse
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 110 111 WB; tested species: human
The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 110 111 WB; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 110 111 WB, IP; tested species: mouse
SNAP23 regulates KCC2 membrane insertion and activity following mZnR/GPR39 activation in hippocampalneurons.
Asraf H, Bogdanovic M, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
iScience (2022) 252: 103751. 110 111 WB; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 110 111 WB, IHC; tested species: mouse
Hyperglycemia-Induced Dysregulated Fusion Intermediates in Insulin-Secreting Cells Visualized by Super-Resolution Microscopy.
Yang G, Li L, Liu Y, Liang K, Wei L, Chen L
Frontiers in cell and developmental biology (2021) 9: 650167. 110 111 WB; tested species: rat
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 110 111 WB; tested species: mouse
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 110 111 WB; KD verified; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 110 111 WB; tested species: mouse
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 110 111 WB; tested species: human
Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes.
Duan MJ, Yan ML, Wang Q, Mao M, Su D, Sun LL, Li KX, Qu Y, Sun Q, Zhang XY, Huang SY, et al.
Cell communication and signaling : CCS (2018) 161: 91. 110 111 WB; tested species: mouse
CRISPR interference-based specific and efficient gene inactivation in the brain.
Zheng Y, Shen W, Zhang J, Yang B, Liu YN, Qi H, Yu X, Lu SY, Chen Y, Xu YZ, Li Y, et al.
Nature neuroscience (2018) 213: 447-454. 110 111 WB; KD verified; tested species: mouse
Analysis of SUMO1-conjugation at synapses.
Daniel JA, Cooper BH, Palvimo JJ, Zhang FP, Brose N, Tirard M
eLife (2017) 6: . 110 111 WB; tested species: mouse
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 110 111 WB
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 110 111 WB; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 111 WB, EM
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 110 111 WB; tested species: mouse
Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus.
Araya-Callís C, Hiemke C, Abumaria N, Flugge G
Psychopharmacology (2012) 2241: 209-22. 110 111 WB; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 110 111 WB
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 110 111 WB, ICC
SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions.
Lam AD, Tryoen-Toth P, Tsai B, Vitale N, Stuenkel EL
Molecular biology of the cell (2008) 192: 485-97. 110 111 WB, IP; tested species: rat
Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
Journal of cell science (2004) 117Pt 10: 1923-35. 110 111 WB, ICC
The interaction between syntaxin 1A and cystic fibrosis transmembrane conductance regulator Cl- channels is mechanistically distinct from syntaxin 1A-SNARE interactions.
Ganeshan R, Di A, Nelson DJ, Quick MW, Kirk KL
The Journal of biological chemistry (2003) 2785: 2876-85. 110 111 WB
The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.
Hatsuzawa K, Lang T, Fasshauer D, Bruns D, Jahn R
The Journal of biological chemistry (2003) 27833: 31159-66. 110 111 WB, ICC
Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.
Varoqueaux F, Sigler A, Rhee JS, Brose N, Enk C, Reim K, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2002) 9913: 9037-42. 110 111 WB
Regulation of Neural Circuit Development by Cadherin-11 Provides Implications for Autism.
Frei JA, Niescier RF, Bridi MS, Durens M, Nestor JE, Kilander MBC, Yuan X, Dykxhoorn DM, Nestor MW, Huang S, Blatt GJ, et al.
eNeuro () 84: . 110 111 WB; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 110 111 WB, IP; tested species: mouse
SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions.
Lam AD, Tryoen-Toth P, Tsai B, Vitale N, Stuenkel EL
Molecular biology of the cell (2008) 192: 485-97. 110 111 WB, IP; tested species: rat
The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 111 WB, ICC; KO verified; tested species: mouse
Altered Distribution of SNARE Proteins in Primary Neurons Exposed to Different Alpha-Synuclein Proteoforms.
Brolin E, Ingelsson M, Bergström J, Erlandsson A
Cellular and molecular neurobiology (2023) : . 110 111 ICC; tested species: mouse
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 110 111 ICC; tested species: rat
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 111 ICC; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 110 111 WB, ICC
Syntaxin1A lateral diffusion reveals transient and local SNARE interactions.
Ribrault C, Reingruber J, Petković M, Galli T, Ziv NE, Holcman D, Triller A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3148: 17590-602. 110 111 ICC; tested species: rat
A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.
Jiang X, Litkowski PE, Taylor AA, Lin Y, Snider BJ, Moulder KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 305: 1798-809. 110 111 ICC
A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.
Barszczewski M, Chua JJ, Stein A, Winter U, Heintzmann R, Zilly FE, Fasshauer D, Lang T, Jahn R
Molecular biology of the cell (2008) 193: 776-84. 110 111 ICC
Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
Journal of cell science (2004) 117Pt 10: 1923-35. 110 111 WB, ICC
The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.
Hatsuzawa K, Lang T, Fasshauer D, Bruns D, Jahn R
The Journal of biological chemistry (2003) 27833: 31159-66. 110 111 WB, ICC
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 110 111 WB, IHC; tested species: mouse
Lipopolysaccharide augments microglial GABA uptake by increasing GABA transporter-1 trafficking and bestrophin-1 expression.
Di Palma M, Catalano M, Serpe C, De Luca M, Monaco L, Kunzelmann K, Limatola C, Conti F, Fattorini G
Glia (2023) 7111: 2527-2540. 110 111 DNA-PAINT; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 111 WB, EM
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Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca2+-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.
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