Synaptic Systems - Syntaxin1A

Cat. No.: 110 118

Amount: 50 µg

Price: $415.00

WB

ICC

IHC

IHC-P

Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca²⁺-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Foletti DL et al., J. Neurosci. (2000)
4 ) Jahn R et al., Annu. Rev. Biochem. (1999)
5 ) Südhof TC et al., Nature (1995)
6 ) Bennett MK et al., Science (1992)
7 ) Inoue A et al., J. Biol. Chem. (1992)
8 ) Hirai Y et al., Cell (1992)
9 ) Barnstable CJ et al., Brain Res. (1985)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 110 118	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: yes (see remarks) ICC: yes gallery IHC: 1 : 500 (see remarks) gallery IHC-P: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb78.3
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to cytoplasmic-domain without membrane anchor of rat Syntaxin 1A. (UniProt Id: P32851)
Reactivity	Reacts with: human (Q16623), rat (P32851), mouse (O35526), mammals, chicken. Other species not tested yet.
Specificity	Specific for syntaxin 1A, no cross-reactivity to syntaxin 1B K.O. validated
Matching control protein/peptide	110-1P
Remarks	The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 78.3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IP: Immunoprecipitates syntaxin 1A and B including complexes with synaptobrevin and SNAP 25. IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	110_118.pdf

Syntaxin1A antibody - 110 118 K.O.