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Syntaxin 1A antibody - 110 118

Syntaxin1 is a plasma membrane SNARE protein essential for synaptic vesicle fusion
Rabbit monoclonal recombinant IgG
Cat. No.: 110 118
Amount: 50 µg
Price: $415.00
Cat. No. 110 118 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: yes (see remarks)
ICC: yes gallery  
IHC: not recommended
IHC-P/FFPE: yes gallery  
Clone Rb78.3
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to cytoplasmic-domain without membrane anchor of rat Syntaxin 1A. (UniProt Id: P32851)
Reactivity Reacts with: human (Q16623), rat (P32851), mouse (O35526).
Other species not tested yet.
Specificity Specific for syntaxin 1A, no cross-reactivity to syntaxin 1B K.O.
Matching control protein/peptide 110-1P
Remarks

The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 78.3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IP: Immunoprecipitates syntaxin 1A and B including complexes with synaptobrevin and SNAP 25.

Data sheet 110_118.pdf
Cat. No.: 110 118
Amount: 50 µg
Price: $415.00
Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca2+-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.