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Synaptophysin1 peptide - 101-0P

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
control peptide
Cat. No.: 101-0P
Amount: 100 µg
Price: $105.00
Cat. No. 101-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of human Synaptophysin (UniProt Id: P08247)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 101 002, 101 004, 101 006
Remarks

This control peptide consists of the synthetic peptide (GPQGAPTSFSNQM) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 101-0p.pdf
Cat. No.: 101-0P
Amount: 100 µg
Price: $105.00
Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.

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