Synaptic Systems - Synaptophysin1

Cat. No.: 101 006

Amount: 200 µl

Price: $370.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 101 006	200 µl antibody, lyophilized. Albumin was added for stabilization. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of human Synaptophysin (UniProt Id: P08247)
Reactivity	Reacts with: human (P08247), rat (P07825), mouse (Q62277). Other species not tested yet.
Matching control protein/peptide	101-0P
Data sheet	101_006.pdf

References for Synaptophysin1 - 101 006

WB (1)
ICC (7)
IHC (7)

G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
Stykel MG, Siripala SV, Soubeyrand E, Coackley CL, Lu P, Camargo S, Thevasenan S, Figueroa GB, So RWL, Stuart E, Panchal R, et al.
Cell reports (2025) 441: 115178. 101 006 WB, ICC; tested species: human,mouse,rat

An In Vitro Assay to Measure Astrocyte-Dependent Synaptic Phagocytosis in Health and Major Depressive Disorder.
Vesga-Jimenez DJ, Vivi E, Di Benedetto B
Methods in molecular biology (Clifton, N.J.) (2025) 2896: 191-201. 101 006 ICC; tested species: rat

Methods for culturing adult CNS neurons reveal a CNS conditioning effect.
van Niekerk EA, Kawaguchi R, Marques de Freria C, Groeniger K, Marchetto MC, Dupraz S, Bradke F, Geschwind DH, Gage FH, Tuszynski MH
Cell reports methods (2022) 27: 100255. 101 006 ICC; tested species: mouse

Comparative chromatin accessibility upon BDNF stimulation delineates neuronal regulatory elements.
Ibarra IL, Ratnu VS, Gordillo L, Hwang IY, Mariani L, Weinand K, Hammarén HM, Heck J, Bulyk ML, Savitski MM, Zaugg JB, et al.
Molecular systems biology (2022) 188: e10473. 101 006 ICC; tested species: human,mouse

Modulating electrophysiology of motor neural networks via optogenetic stimulation during neurogenesis and synaptogenesis.
Pagan-Diaz GJ, Drnevich J, Ramos-Cruz KP, Sam R, Sengupta P, Bashir R
Scientific reports (2020) 101: 12460. 101 006 ICC; tested species: mouse

Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.
Kovács RÁ, Vadászi H, Bulyáki É, Török G, Tóth V, Mátyás D, Kun J, Hunyadi-Gulyás É, Fedor FZ, Csincsi Á, Medzihradszky K, et al.
Frontiers in immunology (2020) 11: 599771. 101 006 ICC, IHC; tested species: mouse

High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons.
Parker SS, Moutal A, Cai S, Chandrasekaran S, Roman MR, Koshy AA, Khanna R, Zinsmaier KE, Mouneimne G
eNeuro () 55: . 101 006 ICC; tested species: mouse

NLRX1 limits inflammatory neurodegeneration in the anterior visual pathway.
Gill AJ, Smith MD, Galleguillos D, Garton T, Mace JW, Gadani SP, Kumar S, Pokharel A, Solem K, Potluri S, Hussein O, et al.
Journal of neuroinflammation (2025) 221: 21. 101 006 IHC; tested species: mouse

Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation.
Garton T, Smith MD, Kesharwani A, Gharagozloo M, Oh S, Na CH, Absinta M, Reich DS, Zack DJ, Calabresi PA
Nature communications (2025) 161: 3481. 101 006 IHC; tested species: mouse

Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis.
Nagumanova A, Seeholzer LR, Di Benedetto B
Methods in molecular biology (Clifton, N.J.) (2025) 2896: 203-214. 101 006 IHC; tested species: rat

Tau association with synaptic mitochondria coincides with energetic dysfunction and excitatory synapse loss in the P301S tauopathy mouse model.
Daniel Estrella L, Trease AJ, Sheldon L, Roland NJ, Fox HS, Stauch KL
Neurobiology of aging (2024) 147: 163-175. 101 006 IHC; tested species: mouse

BDNF-dependent modulation of axonal transport is selectively impaired in ALS.
Tosolini AP, Sleigh JN, Surana S, Rhymes ER, Cahalan SD, Schiavo G
Acta neuropathologica communications (2022) 101: 121. 101 006 IHC; tested species: mouse

Synaptic mitochondrial dysfunction and septin accumulation are linked to complement-mediated synapse loss in an Alzheimer's disease animal model.
Györffy BA, Tóth V, Török G, Gulyássy P, Kovács RÁ, Vadászi H, Micsonai A, Tóth ME, Sántha M, Homolya L, Drahos L, et al.
Cellular and molecular life sciences : CMLS (2020) : . 101 006 IHC; tested species: mouse

Cat. No.: 101 006

Amount: 200 µl

Price: $370.00

References
WB (1)
ICC (7)
IHC (7)

ICC

IHC

IHC-P

Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca²⁺ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.

1 ) Janz R et al., Neuron (1999)
2 ) McMahon HT et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
3 ) Südhof TC et al., Nature (1995)
4 ) Buckley KM et al., J. Cell Biol. (1987)
5 ) Leube RE et al., EMBO J. (1987)
6 ) Südhof TC et al., Science (1987)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Synaptophysin1 antibody - 101 006

References for Synaptophysin1 - 101 006