|Cat. No. 101-0P||
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
|Immunogen||Synthetic peptide corresponding to AA 301 to 313 from human Synaptophysin1 (UniProt Id: P08247)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||101 002, 101 004, 101 006|
This control peptide consists of the synthetic peptide (GPQGAPTSFSNQM) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.