Synaptic Systems

Cat. No.: 107 102

Amount: 200 µl

Price: $355.00

Cat. No. 107 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: yes gallery IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 2 to 14 from rat Rab3a (UniProt Id: P63012)
Reactivity	Reacts with: human (P20336), rat (P63012), mouse (P63011), hamster, cow, zebrafish. Other species not tested yet.
Matching control protein/peptide	107-1P
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 107 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	107_102.pdf

References for Rab3a - 107 102

The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 107 102 WB

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 107 102 WB; tested species: mouse

SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC

Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion.
Schlüter OM, Khvotchev M, Jahn R, Südhof TC
The Journal of biological chemistry (2002) 27743: 40919-29. 107 102 WB, IHC

Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 107 102 WB, IHC

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC

SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC

Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body.
Hruskova B, Trojanova J, Kralikova M, Melichar A, Suchankova S, Bartosova J, Burianova JS, Popelar J, Syka J, Turecek R
Neuroscience letters (2019) : . 107 102 IHC; tested species: rat

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 107 102 IHC; tested species: mouse

Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a.
Rastaldi MP, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, Langer B, Kerjaschki D, Henger A, Blattner SM, Kretzler M, et al.
The American journal of pathology (2003) 1633: 889-99. 107 102 IHC

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 107 102 ELISA

Cat. No.: 107 102

Amount: 200 µl

Price: $355.00

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC

SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC

SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC

ICC

IHC

Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca²⁺ regulated excocytosis and converts it into an constitutive Ca²⁺ independent exocytosis mechanism.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Geppert M et al., Nature (1997)
4 ) Südhof TC et al., Nature (1995)
5 ) Fischer von Mollard G et al., Eur. J. Cell Biol. (1994)
6 ) Chen X et al., J. Biol. Chem. (2003)
7 ) Millar AL et al., Histol. Histopathol. (2002)
8 ) Pavlos NJ et al., J. Mol. Endocrinol. (2001)
9 ) Lin CG et al., Biochem. J. (1997)
10 ) Fischer von Mollard G et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

ICC

IHC

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Rab3a antibody - 107 102

References for Rab3a - 107 102