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Rab3a peptide - 107-1P

Rab3 proteins are the major Rab isoforms in brain and are highly enriched on synaptic vesicles
control peptide
Cat. No.: 107-1P
Amount: 100 µg
Price: $110.00
Cat. No. 107-1P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to AA 2 to 14 from rat Rab3a (UniProt Id: P63012)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 107 102
Remarks

This control peptide consists of the synthetic peptide (ASATDSRYGQKES) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 107-1p.pdf
Cat. No.: 107-1P
Amount: 100 µg
Price: $110.00
Background

Rab3 proteins belong to the Rab family, a subset of the Ras-related superfamily of small monomeric GTPases. There are four known isoforms: Rab3a, Rab3b, Rab3c, and Rab3d (1, 2). Rab3a and Rab3c are primarily found in neuronal and neuroendocrine cells, whereas Rab3b and Rab3d are predominantly expressed in non-neuronal tissues such as the parotid gland, pancreas, mast cells, and adipose tissue (2, 3).
Rab3a, the most abundant and well-characterized isoform, is highly enriched in synaptic vesicles. It regulates vesicle transport, docking, fusion, and Ca²⁺-dependent neurotransmitter release by cycling between a GDP-bound inactive state and a GTP-bound vesicle-associated active state, interacting with other synaptic proteins in the process (1, 2).
Unlike integral membrane proteins of synaptic vesicles, Rab3a and Rab3c are absent from the Golgi complex, preventing immunostaining of the axo-dendritic region, which can occur with proteins such as synaptophysin, synaptobrevin/VAMP, or synaptogyrin (1).
Beyond the nervous system, Rab3a is also expressed in the pancreas, where it is predominantly localized to β-cells of the islets of Langerhans. It plays a crucial role in regulated insulin secretion, while Rab3d is primarily involved in exocrine pancreatic enzyme secretion (3).

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