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Rab3a antibody - 107 102

Rab3 proteins are the major Rab isoforms in brain and are highly enriched on synaptic vesicles
Rabbit polyclonal antiserum
Cat. No.: 107 102
Amount: 200 µl
Price: $360.00
Cat. No. 107 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: yes gallery  
IHC-P: not tested yet
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.

Immunogen Synthetic peptide corresponding to AA 2 to 14 from rat Rab3a (UniProt Id: P63012)
Reactivity Reacts with: human (P20336), rat (P63012), mouse (P63011), hamster, cow, zebrafish.
Other species not tested yet.
Matching control protein/peptide 107-1P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 107 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 107_102.pdf

References for Rab3a - 107 102

The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 107 102 WB
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 107 102 WB; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC
Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion.
Schlüter OM, Khvotchev M, Jahn R, Südhof TC
The Journal of biological chemistry (2002) 27743: 40919-29. 107 102 WB, IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 107 102 WB, IHC
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC
Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body.
Hruskova B, Trojanova J, Kralikova M, Melichar A, Suchankova S, Bartosova J, Burianova JS, Popelar J, Syka J, Turecek R
Neuroscience letters (2019) : . 107 102 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 107 102 IHC; tested species: mouse
Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a.
Rastaldi MP, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, Langer B, Kerjaschki D, Henger A, Blattner SM, Kretzler M, et al.
The American journal of pathology (2003) 1633: 889-99. 107 102 IHC
Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion.
Schlüter OM, Khvotchev M, Jahn R, Südhof TC
The Journal of biological chemistry (2002) 27743: 40919-29. 107 102 WB, IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 107 102 WB, IHC
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 107 102 ELISA
Cat. No.: 107 102
Amount: 200 µl
Price: $360.00
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 107 102 WB
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 107 102 WB; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC
Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion.
Schlüter OM, Khvotchev M, Jahn R, Südhof TC
The Journal of biological chemistry (2002) 27743: 40919-29. 107 102 WB, IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 107 102 WB, IHC
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 102 WB, ICC
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 107 102 WB, ICC
Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body.
Hruskova B, Trojanova J, Kralikova M, Melichar A, Suchankova S, Bartosova J, Burianova JS, Popelar J, Syka J, Turecek R
Neuroscience letters (2019) : . 107 102 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 107 102 IHC; tested species: mouse
Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a.
Rastaldi MP, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, Langer B, Kerjaschki D, Henger A, Blattner SM, Kretzler M, et al.
The American journal of pathology (2003) 1633: 889-99. 107 102 IHC
Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion.
Schlüter OM, Khvotchev M, Jahn R, Südhof TC
The Journal of biological chemistry (2002) 27743: 40919-29. 107 102 WB, IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 107 102 WB, IHC
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 107 102 ELISA
Background

Rab3 proteins belong to the Rab family, a subset of the Ras-related superfamily of small monomeric GTPases. There are four known isoforms: Rab3a, Rab3b, Rab3c, and Rab3d (1, 2). Rab3a and Rab3c are primarily found in neuronal and neuroendocrine cells, whereas Rab3b and Rab3d are predominantly expressed in non-neuronal tissues such as the parotid gland, pancreas, mast cells, and adipose tissue (2, 3).
Rab3a, the most abundant and well-characterized isoform, is highly enriched in synaptic vesicles. It regulates vesicle transport, docking, fusion, and Ca²⁺-dependent neurotransmitter release by cycling between a GDP-bound inactive state and a GTP-bound vesicle-associated active state, interacting with other synaptic proteins in the process (1, 2).
Unlike integral membrane proteins of synaptic vesicles, Rab3a and Rab3c are absent from the Golgi complex, preventing immunostaining of the axo-dendritic region, which can occur with proteins such as synaptophysin, synaptobrevin/VAMP, or synaptogyrin (1).
Beyond the nervous system, Rab3a is also expressed in the pancreas, where it is predominantly localized to β-cells of the islets of Langerhans. It plays a crucial role in regulated insulin secretion, while Rab3d is primarily involved in exocrine pancreatic enzyme secretion (3).