Synaptic Systems

Cat. No.: 107 111

Amount: 100 µg

Price: $420.00

Cat. No. 107 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 1000 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 2000 up to 1 : 3000 gallery DNA-PAINT: yes (see remarks) EM: yes ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	42.2
Subtype	IgG1 (κ light chain)
Immunogen	Full length rat recombinant Rab3a (UniProt Id: P63012)
Epitop	AA 191 to 220 from rat Rab3a (UniProt Id: P63012)
Reactivity	Reacts with: human (P20336), rat (P63012), mouse (P63011), mammals. Other species not tested yet.
Specificity	K.O. validated PubMed: 12244319
Remarks	DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614). ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 107 102 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	107_111.pdf

References for Rab3a - 107 111

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 107 111 WB; tested species: human

zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1.
Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ
JACC. Basic to translational science (2023) 85: 518-542. 107 111 WB; tested species: mouse

Glutamatergic synapses from the insular cortex to the basolateral amygdala encode observational pain.
Zhang MM, Geng AQ, Chen K, Wang J, Wang P, Qiu XT, Gu JX, Fan HW, Zhu DY, Yang SM, Chen QY, et al.
Neuron (2022) 11012: 1993-2008.e6. 107 111 WB; tested species: mouse

Maternal stress programs accelerated aging of the basal ganglia motor system in offspring.
Marrocco J, Verhaeghe R, Bucci D, Di Menna L, Traficante A, Bouwalerh H, Van Camp G, Ghiglieri V, Picconi B, Calabresi P, Ravasi L, et al.
Neurobiology of stress (2020) 13: 100265. 107 111 WB; tested species: rat

Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 107 111 WB, IHC; tested species: mouse

The reduction in glutamate release is predictive of cognitive and emotional alterations that are corrected by the positive modulator of AMPA receptors S 47445 in perinatal stressed rats.
Morley-Fletcher S, Zuena AR, Mairesse J, Gatta E, Van Camp G, Bouwalerh H, Riozzi B, Battaglia G, Pittaluga A, Olivero G, Mocaer E, et al.
Neuropharmacology (2018) 135: 284-296. 107 111 WB; tested species: rat

The Prion Protein Regulates Synaptic Transmission by Controlling the Expression of Proteins Key to Synaptic Vesicle Recycling and Exocytosis.
Peggion C, Stella R, Chemello F, Massimino ML, Arrigoni G, Cagnin S, Biancotto G, Franchin C, Sorgato MC, Bertoli A
Molecular neurobiology (2018) : . 107 111 WB; tested species: mouse

SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 107 111 WB; tested species: monkey

Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 107 111 WB; tested species: mouse

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 107 111 WB

OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency.
Ullrich M, Weber M, Post AM, Popp S, Grein J, Zechner M, Guerrero González H, Kreis A, Schmitt AG, Üçeyler N, Lesch KP, et al.
Molecular psychiatry (2017) : . 107 111 WB

Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 107 111 WB; tested species: mouse

Generation of a novel human cytomegalovirus bacterial artificial chromosome tailored for transduction of exogenous sequences.
Close WL, Bhandari A, Hojeij M, Pellett PE
Virus research (2017) 242: 66-78. 107 111 WB; tested species: human

JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Caswell PT, Dickens M
Molecular and cellular biochemistry (2017) : . 107 111 WB, ICC; tested species: rat

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sheehan P, Zhu M, Beskow A, Vollmer C, Waites CL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3633: 8668-86. 107 111 WB; tested species: rat

A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins.
Rutkowska-Wlodarczyk I, Aller MI, Valbuena S, Bologna JC, Prézeau L, Lerma J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3513: 5171-9. 107 111 WB

The effects of antidepressant treatment in prenatally stressed rats support the glutamatergic hypothesis of stress-related disorders.
Marrocco J, Reynaert ML, Gatta E, Gabriel C, Mocaër E, Di Prisco S, Merega E, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 346: 2015-24. 107 111 WB; tested species: rat

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 107 111 WB

BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 107 111 WB, ICC; tested species: rat

Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 107 111 WB

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 107 111 WB; tested species: mouse

Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.
Bonnet SA, Akad DS, Samaddar T, Liu Y, Huang X, Dong Y, Schlüter OM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3333: 13398-409. 107 111 WB; tested species: mouse

Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 107 111 WB

Myosin5a tail associates directly with Rab3A-containing compartments in neurons.
Wöllert T, Patel A, Lee YL, Provance DW, Vought VE, Cosgrove MS, Mercer JA, Langford GM
The Journal of biological chemistry (2011) 28616: 14352-61. 107 111 WB, ICC; tested species: mouse

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 107 111 WB, IP, ICC

Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 107 111 WB; tested species: human

Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse.
Yang S, Farias M, Kapfhamer D, Tobias J, Grant G, Abel T, Bućan M
Genes, brain, and behavior (2007) 61: 77-96. 107 111 WB, IP

Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 107 111 WB; tested species: mouse

Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 107 111 WB; tested species: rat

Mutations in Rab3a alter circadian period and homeostatic response to sleep loss in the mouse.
Kapfhamer D, Valladares O, Sun Y, Nolan PM, Rux JJ, Arnold SE, Veasey SC, Bućan M
Nature genetics (2002) 322: 290-5. 107 111 WB; KO verified; tested species: mouse

SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.
Chamberlain LH, Burgoyne RD, Gould GW
Proceedings of the National Academy of Sciences of the United States of America (2001) 9810: 5619-24. 107 111 WB; tested species: rat

Rabphilin knock-out mice reveal that rabphilin is not required for rab3 function in regulating neurotransmitter release.
Schlüter OM, Schnell E, Verhage M, Tzonopoulos T, Nicoll RA, Janz R, Malenka RC, Geppert M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (1999) 1914: 5834-46. 107 111 WB, IHC

Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C.
Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC
Neuron (1994) 134: 885-98. 107 111 WB, IHC

Structure of the murine rab3A gene: correlation of genomic organization with antibody epitopes.
Baumert M, Fischer von Mollard G, Jahn R, Südhof TC
The Biochemical journal (1993) 293 ( Pt 1): 157-63. 107 111 WB

Association of Rab3A with synaptic vesicles at late stages of the secretory pathway.
Matteoli M, Takei K, Cameron R, Hurlbut P, Johnston PA, Südhof TC, Jahn R, De Camilli P
The Journal of cell biology (1991) 1153: 625-33. 107 111 ICC, IHC, WB, EM; tested species: rat,frog

Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates.
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P
Nature communications (2023) 141: 455. 107 111 ICC; tested species: mouse

Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 107 111 ICC; tested species: mouse

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 111 ICC; tested species: rat

Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging.
Neukam M, Sala P, Brunner AD, Ganß K, Palladini A, Grzybek M, Topcheva O, Vasiljević J, Broichhagen J, Johnsson K, Kurth T, et al.
Cell reports (2024) 433: 113836. 107 111 IHC; tested species: mouse

Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 107 111 IHC; tested species: rat

Tissue multicolor STED nanoscopy of presynaptic proteins in the calyx of Held.
Kempf C, Staudt T, Bingen P, Horstmann H, Engelhardt J, Hell SW, Kuner T
PloS one (2013) 84: e62893. 107 111 IHC; tested species: rat

Differential distribution of glycine receptor subtypes at the rat calyx of Held synapse.
Hruskova B, Trojanova J, Kulik A, Kralikova M, Pysanenko K, Bures Z, Syka J, Trussell LO, Turecek R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3247: 17012-24. 107 111 IHC

Impaired activity-dependent plasticity of quantal amplitude at the neuromuscular junction of Rab3A deletion and Rab3A earlybird mutant mice.
Wang X, Wang Q, Yang S, Bucan M, Rich MM, Engisch KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3110: 3580-8. 107 111 IHC

Mice lacking alpha-synuclein display functional deficits in the nigrostriatal dopamine system.
Abeliovich A, Schmitz Y, Fariñas I, Choi-Lundberg D, Ho WH, Castillo PE, Shinsky N, Verdugo JM, Armanini M, Ryan A, Hynes M, et al.
Neuron (2000) 251: 239-52. 107 111 IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 107 111 DNA-PAINT; tested species: rat

A novel flat-embedding method to prepare ultrathin cryosections from cultured cells in their in situ orientation.
Oorschot V, de Wit H, Annaert WG, Klumperman J
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2002) 508: 1067-80. 107 111 EM

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 107 111 ELISA

Cat. No.: 107 111

Amount: 100 µg

Price: $420.00

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 107 111 WB; tested species: mouse

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 107 111 ICC; tested species: rat

ICC

IHC

IHC-P

Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca²⁺ regulated excocytosis and converts it into an constitutive Ca²⁺ independent exocytosis mechanism.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Geppert M et al., Nature (1997)
4 ) Südhof TC et al., Nature (1995)
5 ) Fischer von Mollard G et al., Eur. J. Cell Biol. (1994)
6 ) Chen X et al., J. Biol. Chem. (2003)
7 ) Millar AL et al., Histol. Histopathol. (2002)
8 ) Pavlos NJ et al., J. Mol. Endocrinol. (2001)
9 ) Lin CG et al., Biochem. J. (1997)
10 ) Fischer von Mollard G et al., J. Biol. Chem. (1994)

Protocols

Standard protocol

ICC

IHC

IHC-P

ELISA

Membrane proteins

Other products for this target

Rab3a antibody - 107 111 K.O.

References for Rab3a - 107 111