Cat. No.: 180-1P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 180-1P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 828 to 979 from rat GluK5 (UniProt Id: Q63273) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 180 103 |
| Remarks |
This control protein consists of the recombinant protein (aa 828 - 979 of rat GluK 5) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 180-1p.pdf |
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Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN, and kainate/GluK receptors (KARs) (1, 2).
mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins (3). KARs can be found at pre- and postsynaptic sites and are composed of five different subunits: GluK1, GluK2 and GluK3 can form homomeric receptors whereas GluK4 and GluK5 form heteromeric receptors with GluK1-3 (4).