Synaptic Systems

Cat. No.: 180 103

Amount: 50 µg

Price: $375.00

Cat. No. 180 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 828 to 979 from rat GluK5 (UniProt Id: Q63273)
Reactivity	Reacts with: rat (Q63273), mouse (Q61626). Other species not tested yet.
Matching control protein/peptide	180-1P
Remarks	IHC-Fr: Antibody requires Clarke liquid fixation (1 vol. acetic acid, 6 vol. ethanol, 15 min at RT).
Data sheet	180_103.pdf

References for GluK5 - 180 103

Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 180 103 WB

Localization of kainate receptors in inner and outer hair cell synapses.
Fujikawa T, Petralia RS, Fitzgerald TS, Wang YX, Millis B, Morgado-Díaz JA, Kitamura K, Kachar B
Hearing research (2014) 314: 20-32. 180 103 IHC; tested species: rat

Presenilin and APP Regulate Synaptic Kainate Receptors.
Barthet G, Moreira-de-Sá A, Zhang P, Deforges S, Castanheira J, Gorlewicz A, Mulle C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9253-9262. 180 103 IHC-Fr; tested species: mouse

Cat. No.: 180 103

Amount: 50 µg

Price: $375.00

IHC-Fr

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN, and kainate/GluK receptors (KARs) (1, 2).
mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins (3). KARs can be found at pre- and postsynaptic sites and are composed of five different subunits: GluK1, GluK2 and GluK3 can form homomeric receptors whereas GluK4 and GluK5 form heteromeric receptors with GluK1-3 (4).

1 ) Traynelis SF et al., Pharmacol Rev (2010)
2 ) Collingridge GL et al., Neuropharmacology (2009)
3 ) Bass BL et al., RNA (1997)
4 ) Kumar J et al., Neuron (2011)
5 ) Pinheiro PS et al., Proc. Natl. Acad. Sci. U.S.A. (2007)
6 ) Jaskolski F et al., J. Biol. Chem. (2005)
7 ) Hamassaki-Britto DE et al., J. Neurosci. (1993)
8 ) Strutz-Seebohm N et al., Cell. Physiol. Biochem. (2006)
9 ) Coussen F et al., Neuron (2005)
10 ) Ullal G et al., Epilepsia (2005)

Protocols

IHC-Fr

OTHER

Preabsorbtion

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GluK5 antibody - 180 103

References for GluK5 - 180 103