Cat. No. 198 111 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications | |
Clone | 26H1D1 |
Subtype | IgG3 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320) |
Epitop |
AA 3 to 17 from mouse GAD2 (UniProt Id: P48320) |
Reactivity |
Reacts with: rat (Q05683), mouse (P48320). Other species not tested yet. |
Specificity | Specific for GAD 2 / GAD 65 |
Matching control protein/peptide | 198-1P |
Remarks |
WB: This antibody is less sensitive than the rabbit antibody cat. no. 198 102. |
Data sheet | 198_111.pdf |
The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.