Synaptic Systems

Cat. No.: 198 103

Amount: 50 µg

Price: $460.00

Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 103 WB

Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 198 103 IHC

WB

ICC

IHC

IHC-P

Background

The glutamic acid decarboxylases GAD1 and GAD2, also referred to as GAD67, and GAD65 respectively, synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. Therefore, GADs are widely used markers for the GABAergic system (1). The hydrophilic GAD1 can heterodimerize with the membrane achored GAD2 and a part of GAD1 is targeted to inhibitory nerve terminals by this mechanism (2). Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule (3).
GAD1 and 2 also occur in rodent pancreatic islets of Langerhans, whereas human islets mainly express GAD2 which has been identified as a major autoantigen in type 1 diabetes (3).

1 ) Tamamaki N et al., J. Comp. Neurol. (2003)
2 ) Kanaani J et al., J. Biol. Chem. (1999)
3 ) Kim J et al., Diabetes (1993)

Protocols

WB

IP

Standard protocol

ICC

IHC

IHC-P

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Cat. No. 198 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 2000 gallery IHC: 1 : 1000 gallery IHC-P: 1 : 1000 gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Reactivity	Reacts with: rat (P18088), mouse (P48318). Other species not tested yet.
Specificity	Specific for GAD 2 / GAD 65.
Matching control protein/peptide	198-1P
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 198 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	198_103.pdf

GAD2 (GAD65) antibody - 198 103

References for GAD2 - 198 103