Cat. No.: 214 005
Amount: 50 µg
Price:
$465.00
Cat. No. 214 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 200 up to 1 : 500 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery |
Immunogen | Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937) |
Reactivity |
Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow. Other species not tested yet. |
Matching control protein/peptide | 214-0P |
Data sheet | 214_005.pdf |
Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.