Cat. No.: 152-3P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 152-3P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 152 303, 152 305, 152 311, 152 313 |
| Remarks |
This control protein consists of the recombinant protein (aa 2056-2336 of rat Ca2+ α-1B) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 152-3p.pdf |
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Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).