Cat. No.: 152 311
Amount: 100 µg
Price:
$420.00
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| Cat. No. 152 311 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet |
| Clone | 163E3 |
| Subtype | IgG2b (κ light chain) |
| Immunogen | Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294) |
| Epitop |
AA 2074 to 2354 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294) |
| Reactivity |
Reacts with: rat (Q02294), mouse. Other species not tested yet. |
| Matching control protein/peptide | 152-3P |
| Remarks |
WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel N-type alpha-1B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
| Data sheet | 152_311.pdf |
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