Synaptic Systems - Ca2+ channel N-type alpha-1B

Cat. No.: 152 311

Amount: 100 µg

Price: $415.00

Cat. No. 152 311	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	163E3
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294)
Epitop	AA 2074 to 2354 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294)
Reactivity	Reacts with: rat (Q02294), mouse. Other species not tested yet.
Matching control protein/peptide	152-3P
Remarks	WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel N-type alpha-1B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	152_311.pdf

References for Ca2+ channel N-type alpha-1B - 152 311

CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 152 311 WB, ICC; tested species: rat

Interactions of Rabconnectin-3 with Cav2 calcium channels.
Gandini MA, Souza IA, Fan J, Li K, Wang D, Zamponi GW
Molecular brain (2019) 121: 62. 152 311 ICC; tested species: mouse

RIM-BP2 regulates Ca2+ channel abundance and neurotransmitter release at hippocampal mossy fiber terminals.
Miyano R, Sakamoto H, Hirose K, Sakaba T
eLife (2024) 12: . 152 311 IHC-Fr; tested species: mouse

Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus.
Midorikawa M, Sakamoto H, Nakamura Y, Hirose K, Miyata M
Cell reports (2024) 4310: 114770. 152 311 IHC-Fr; tested species: mouse

Cat. No.: 152 311

Amount: 100 µg

Price: $415.00

Interactions of Rabconnectin-3 with Cav2 calcium channels.
Gandini MA, Souza IA, Fan J, Li K, Wang D, Zamponi GW
Molecular brain (2019) 121: 62. 152 311 ICC; tested species: mouse

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Davies JN et al., Biochem. Biophys. Res. Commun. (2011)
2 ) Scheuber A et al., J. Neurosci. (2004)
3 ) Lin Y et al., J. Neurophysiol. (2004)
4 ) Hell JW et al., J. Biol. Chem. (1994)
5 ) Dubel SJ et al., Proc. Natl. Acad. Sci. U.S.A. (1992)
6 ) Snutch TP et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

OTHER

Preabsorbtion

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Ca2+ channel N-type alpha-1B antibody - 152 311

References for Ca2+ channel N-type alpha-1B - 152 311