Synaptic Systems - Ca2+ channel N-type alpha-1B

Cat. No.: 152 305

Amount: 50 µg

Price: $460.00

Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration (2022) 111: 31. 152 305 ICC; tested species: mouse

BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).
Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S
Frontiers in molecular neuroscience (2017) 10: 346. 152 305 ICC; tested species: mouse

WB

IHC

EM

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Davies JN et al., Biochem. Biophys. Res. Commun. (2011)
2 ) Scheuber A et al., J. Neurosci. (2004)
3 ) Lin Y et al., J. Neurophysiol. (2004)
4 ) Hell JW et al., J. Biol. Chem. (1994)
5 ) Dubel SJ et al., Proc. Natl. Acad. Sci. U.S.A. (1992)
6 ) Snutch TP et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

WB

IHC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 152 305	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294)
Reactivity	Reacts with: rat (Q02294), mouse (O55017). Other species not tested yet.
Matching control protein/peptide	152-3P
Remarks	WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel N-type alpha-1B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires mild fixation.
Data sheet	152_305.pdf

Ca2+ channel N-type alpha-1B antibody - 152 305

References for Ca2+ channel N-type alpha-1B - 152 305