Synaptic Systems - Ca2+ channel N-type alpha-1B

Cat. No.: 152 313

Amount: 50 µg

Price: $375.00

Cat. No. 152 313	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294)
Reactivity	Reacts with: rat (Q02294), mouse (O55017). Other species not tested yet.
Specificity	K.D. validated PubMed: 29198756
Matching control protein/peptide	152-3P
Remarks	IHC: This antibody requires mild fixation.
Data sheet	152_313.pdf

References for Ca2+ channel N-type alpha-1B - 152 313

CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 152 313 WB, ICC; KD verified; tested species: rat

Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 152 313 ICC; tested species: human,mouse

Cat. No.: 152 313

Amount: 50 µg

Price: $375.00

IHC

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Davies JN et al., Biochem. Biophys. Res. Commun. (2011)
2 ) Scheuber A et al., J. Neurosci. (2004)
3 ) Lin Y et al., J. Neurophysiol. (2004)
4 ) Hell JW et al., J. Biol. Chem. (1994)
5 ) Dubel SJ et al., Proc. Natl. Acad. Sci. U.S.A. (1992)
6 ) Snutch TP et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

IHC

OTHER

Preabsorbtion

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Ca2+ channel N-type alpha-1B antibody - 152 313 K.D.

References for Ca2+ channel N-type alpha-1B - 152 313