|Cat. No. 474 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery
Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 100 up to 1 : 2500 (see remarks) gallery
|Immunogen||Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-1. (UniProt Id: P39447)|
Reacts with: mouse (P39447), rat (A0A0G2K2P5), dog (O97758).
Other species not tested yet.
|Specificity||Specific for Tight junction protein ZO-1|
|Matching control protein/peptide||474-0P|
IHC-Fr: Methanol fixation is recommended.
ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein with a molecular mass of 220 kDa, located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells. (1) Two isoforms, ZO-1α− and ZO-1α+, have been described, which, as a result of alternative RNA splicing, differ by an internal 80 amino acid sequence. (2)
ZO-1 binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin. (3)