Cat. No.: 474 005
Amount: 50 µg
Price:
$460.00
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| Cat. No. 474 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-1. (UniProt Id: P39447) |
| Reactivity |
Reacts with: mouse (P39447), rat (A0A0G2K2P5), dog (O97758). Other species not tested yet. |
| Matching control protein/peptide | 474-0P |
| Remarks |
IHC-Fr: Methanol fixation is recommended. |
| Data sheet | 474_005.pdf |
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ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein with a molecular mass of 220 kDa, located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells. (1) Two isoforms, ZO-1α− and ZO-1α+, have been described, which, as a result of alternative RNA splicing, differ by an internal 80 amino acid sequence. (2)
ZO-1 binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin. (3)