TECPR1 antibody - 458 003

TECPR1 is a tethering factor involved in autophagy

Rabbit polyclonal purified antibody

Cat. No.: 458 003

Amount: 50 µg

Price: $380.00

Cat. No. 458 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (AP-staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1115 to 1134 from rat Tecpr1 (UniProt Id: Q3ZBA0)
Reactivity	Reacts with: rat (Q3ZBA0). Other species not tested yet.
Matching control protein/peptide	458-0P
Remarks	WB: Detects endogenous levels of TECPR1 in brain samples.
Data sheet	458_003.pdf

Cat. No.: 458 003

Amount: 50 µg

Price: $380.00

Background

Tectonin beta-propeller repeat-containing protein 1 (TECPR1) consists of two WD-repeat domains (TR), two dysferlin domains, an unstructured region and a PH domain (1). It is involved in autophagy by promoting autophagosome fusion with lysosomes (2) by associating with the ATG5-ATG12 conjugate and phosphatidylinositol-3-phosphate (PtdIns3P) present at the surface of autophagosomes (3). TECPR1 has also been shown to participate in selective autophagy against bacterial pathogens (4) and aggrephagy of protein aggregats that accompany neurodegenarative diseases (5).

1 ) Jeynov B et al., FEBS Lett (2006)
2 ) Wetzel L et al., Nat Commun (2020)
3 ) Chen D et al., Mol Cell (2012)
4 ) Ogawa M et al., Cell Host Microbe (2011)

Protocols

OTHER

Preabsorbtion

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