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AiF - 300-0P

An oxidoreductase in the inner mitochondrial membrane
control peptide
Cat. No.: 300-0P
Amount: 100 µg
Price: $90.00
Cat. No. 300-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Immunogen Synthetic peptide corresponding to AA 514 to 529 from mouse AiF (UniProt Id: Q9Z0X1)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 300 003
Remarks

This control peptide consists of the synthetic peptide (aa 514-529 in mouse AiF) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 300-0p.pdf
Cat. No.: 300-0P
Quantity: 100 µg
Price: $90.00
Background
In healthy cells the apoptosis-inducing factor AiF, also referred to as PDCD 8, localizes to the inner membrane of mitochondria where it functions as an oxidoreductase. After permeabilization of the outer mitochondrial membrane, which is common to apoptotic pathways, AiF is released from mitochondria and translocated to the nucleus. There it contributes to apoptotic chromatin condensation and DNA degradation.
Protocols