AiF peptide - 300-0P

AiF is an oxidoreductase in the inner mitochondrial membrane

control peptide

Cat. No.: 300-0P

Amount: 100 µg

Price: $105.00

Cat. No. 300-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 514 to 529 from mouse AiF (UniProt Id: Q9Z0X1)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	300 003
Remarks	This control peptide consists of the synthetic peptide (aa 514-529 in mouse AiF) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	300-0p.pdf

Cat. No.: 300-0P

Amount: 100 µg

Price: $105.00

Background

In healthy cells the apoptosis-inducing factor AiF, also referred to as PDCD 8, localizes to the inner membrane of mitochondria where it functions as an oxidoreductase. After permeabilization of the outer mitochondrial membrane, which is common to apoptotic pathways, AiF is released from mitochondria and translocated to the nucleus. There it contributes to apoptotic chromatin condensation and DNA degradation.

1 ) Öxler EM et al., Apoptosis (2012)
2 ) Daugas E et al., FEBS Lett. (2000)
3 ) Daugas E et al., FASEB J. (2000)

Protocols

OTHER

Preadsorbtion

Other products for this target