AiF antibody - 300 003

AiF is an oxidoreductase in the inner mitochondrial membrane

Rabbit polyclonal purified antibody

Cat. No.: 300 003

Amount: 50 µg

Price: $375.00

Cat. No. 300 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 514 to 529 from mouse AiF (UniProt Id: Q9Z0X1)
Reactivity	Reacts with: human, rat, mouse (Q9Z0X1). Other species not tested yet.
Specificity	Specific for AiF.
Matching control protein/peptide	300-0P
Data sheet	300_003.pdf

Cat. No.: 300 003

Amount: 50 µg

Price: $375.00

ICC

Background

In healthy cells the apoptosis-inducing factor AiF, also referred to as PDCD 8, localizes to the inner membrane of mitochondria where it functions as an oxidoreductase. After permeabilization of the outer mitochondrial membrane, which is common to apoptotic pathways, AiF is released from mitochondria and translocated to the nucleus. There it contributes to apoptotic chromatin condensation and DNA degradation.

1 ) Öxler EM et al., Apoptosis (2012)
2 ) Daugas E et al., FEBS Lett. (2000)
3 ) Daugas E et al., FASEB J. (2000)

Protocols

ICC

OTHER

Preabsorbtion

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