Synaptic Systems - Cre-recombinase

Cat. No.: 257 005

Amount: 50 µg

Price: $465.00

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Cat. No. 257 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length recombinant Cre-recombinase from Bacteriophage P1. (UniProt Id: P06956)
Specificity	Recognizes Cre- and iCre-recombinase.
Data sheet	Data Sheet 257_005 Safety Data Sheet (SDS)

References for Cre-recombinase - 257 005

Identification of DARPP-32 as a novel sleep regulator in physiological conditions and experimental Parkinsonism.
Pisanò CA, Santino ML, Russotto A, Fisone G
iScience (2026) 293: 114882. 257 005 IHC; tested species: mouse

Oxytocin attenuates fear learning via enhancing somatostatin interneuron-mediated local GABAergic inhibition in the prelimbic cortex.
Xu H, Li Y, Cui X, Zhang Y, Wu X, Zhuo SS, He M, Xiao L
Science advances (2026) 1224: eaef8400. 257 005 IHC; tested species: mouse

Matrix-biased excitatory and inhibitory inputs to the striatum involving external segment of the globus pallidus.
Karube F, Kobayashi K, Fujiyama F
Frontiers in cellular neuroscience (2025) 19: 1706469. 257 005 IHC; tested species: rat

Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact.
Toma K, Zhao M, Zhang S, Wang F, Graham HK, Zou J, Modgil S, Shang WH, Tsai NY, Cai Z, Liu L, et al.
Cell (2024) 18711: 2767-2784.e23. 257 005 IHC; tested species: mouse

Cat. No.: 257 005

Amount: 50 µg

Price: $465.00

ICC
(1 img)

IHC
(2 img)

IHC-P (FFPE)
(1 img)

Background

Cre-Recombinase is a site-specific DNA recombinase derived from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites, enabling precise deletion, inversion, or activation of DNA sequences. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state [1].
In experimental biology, Cre is commonly expressed under tissue-, cell-, or temporally specific promoters, allowing conditional manipulation of genes in transgenic and knockout mouse models [2, 3]. Inducible variants such as Cre-ERT are fused to a modified estrogen receptor domain and can be activated by tamoxifen, providing temporal control of recombination [4].

1 ) Lanza AM et al., Biotechnol J (2012)
2 ) Nagy A et al., Genesis (2000)
3 ) Brault V et al., Handb Exp Pharmacol (2007)
4 ) Feil R et al., Proc Natl Acad Sci U S A (1996)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cre-recombinase antibody - 257 005

References for Cre-recombinase - 257 005