|Cat. No. 234 009||50 µg purified IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Before storing at -20°C add 1 vol of glycerol.|
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery
|Subtype||IgY (λ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)|
Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008).
Other species not tested yet.
|Specificity||Specific for IBA 1|
|Matching control protein/peptide||234-0P|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).