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IBA1 - 234 009

Marker for macrophages and microglia
Monoclonal Recombinant chicken purified IgY
Cat. No.: 234 009
Amount: 50 µg
Price: $415.00
Cat. No. 234 009 50 µg purified IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Before storing at -20°C add 1 vol of glycerol.
Applications WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery  
Clone Ch311H9
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 234_009.pdf
Cat. No.: 234 009
Quantity: 50 µg
Price: $415.00
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).