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IBA 1 - 234-0P

A calcium binding protein specific for microglia
control peptide
Cat. No.: 234-0P
Amount: 100 µg
Price: 90.00 €
Cat. No. 234-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Immunogen Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 234 003, 234 004, 234 006
Remarks This control peptide consists of the synthetic peptide (aa 134 - 147 in rat IBA 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet 234-0p.pdf
Cat. No.: 234-0P
Quantity: 100 µg
Price: 90.00 €
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3). 

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