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IBA1 - 234 011

Marker for macrophages and microglia
Monoclonal mouse purified IgG
Cat. No.: 234 011
Amount: 100 µg
Price: $415.00
Cat. No. 234 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 500 up to 1 : 1000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 2000 gallery  
Clone 311H9H4
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1
Matching control protein/peptide 234-0P
Data sheet 234_011.pdf
Cat. No.: 234 011
Quantity: 100 µg
Price: $415.00
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).