Cat. No.: 234 011
Amount: 100 µg
Price:
$415.00
| Cat. No. 234 011 | 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. |
| Applications |
WB: 1 : 500 up to 1 : 1000 gallery IP: not tested yet ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 up to 1 : 2000 gallery IHC-P/FFPE: 1 : 100 up to 1 : 2000 gallery |
| Clone | 311H9H4 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 134 to 147 from rat IBA1 (UniProt Id: P55009) |
| Reactivity |
Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008). Other species not tested yet. |
| Specificity | Specific for IBA 1 |
| Matching control protein/peptide | 234-0P |
| Data sheet | 234_011.pdf |
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Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).