Synaptic Systems

Cat. No.: 234 308

Amount: 50 µg

Price: $430.00

Cat. No. 234 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery
Clone	Gp311H9
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity	Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008), monkey. Other species not tested yet.
Matching control protein/peptide	234-0P
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	234_308.pdf

References for IBA1 - 234 308

WB (1)
ICC (4)
IHC (19)
IHC-P (3)

Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization.
Wang N, Li F, Du J, Hao J, Wang X, Hou Y, Luo Z
Journal of inflammation research (2024) 17: 1281-1293. 234 308 WB, IHC; tested species: rat

Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.
Park JC, Han JW, Lee W, Kim J, Lee SE, Lee D, Choi H, Han J, Kang YJ, Diep YN, Cho H, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400064. 234 308 ICC, IHC; tested species: human,mouse

Rapid phagosome isolation enables unbiased multiomic analysis of human microglial phagosomes.
Wogram E, Sümpelmann F, Dong W, Rawat E, Fernández Maestre I, Fu D, Braswell B, Khalil A, Buescher JM, Mittler G, Borner GHH, et al.
Immunity (2024) 579: 2216-2231.e11. 234 308 ICC; tested species: human

The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 234 308 ICC, IHC; tested species: mouse

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 234 308 ICC; tested species: mouse

Postnatal downregulation of Fmr1 in microglia promotes microglial reactivity and causes behavioural alterations in female mice.
Hooshmandi M, Ho-Tieng D, Lister KC, Cai W, Wong C, Brown N, Fan J, Hovhannisyan V, Uttam S, Prager-Khoutorsky M, Sonenberg N, et al.
Molecular autism (2025) 161: 17. 234 308 IHC; tested species: mouse

25-Hydroxycholesterol modulates microglial function and exacerbates Alzheimer's disease pathology: mechanistic insights and therapeutic potential of cholesterol esterification inhibition.
Choi H, Kim HJ, Lee SE, Song HH, Kim J, Han J, Jeong JH, Lee DY, Chang S, Mook-Jung I
Journal of neuroinflammation (2025) 221: 50. 234 308 IHC; tested species: mouse

Inhibition of repulsive guidance molecule A ameliorates diabetes-induced cognitive decline and hippocampal neurogenesis impairment in mice.
Uno H, Itokazu T, Yamashita T
Communications biology (2025) 81: 263. 234 308 IHC; tested species: mouse

Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 308 IHC; tested species: mouse

Homeostatic microglia initially seed and activated microglia later reshape amyloid plaques in Alzheimer's Disease.
Baligács N, Albertini G, Borrie SC, Serneels L, Pridans C, Balusu S, De Strooper B
Nature communications (2024) 151: 10634. 234 308 IHC; tested species: mouse

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19.
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, et al.
Cell host & microbe (2024) : . 234 308 IHC; tested species: human,mouse

Myocardial infarction augments sleep to limit cardiac inflammation and damage.
Huynh P, Hoffmann JD, Gerhardt T, Kiss MG, Zuraikat FM, Cohen O, Wolfram C, Yates AG, Leunig A, Heiser M, Gaebel L, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse

Fibrin drives thromboinflammation and neuropathology in COVID-19.
Ryu JK, Yan Z, Montano M, Sozmen EG, Dixit K, Suryawanshi RK, Matsui Y, Helmy E, Kaushal P, Makanani SK, Deerinck TJ, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse

hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.
Zare A, Salehi S, Bader J, Schneider C, Fischer U, Veh A, Arampatzi P, Mann M, Briese M, Sendtner M
Nature communications (2024) 151: 7430. 234 308 IHC; tested species: mouse

IRF8 and MAFB drive distinct transcriptional machineries in different resident macrophages of the central nervous system.
Yamasaki A, Imanishi I, Tanaka K, Ohkawa Y, Tsuda M, Masuda T
Communications biology (2024) 71: 896. 234 308 IHC; tested species: mouse

Nigrostriatal degeneration determines dynamics of glial inflammatory and phagocytic activity.
Ayerra L, Abellanas MA, Basurco L, Tamayo I, Conde E, Tavira A, Trigo A, Vidaurre C, Vilas A, San Martin-Uriz P, Luquin E, et al.
Journal of neuroinflammation (2024) 211: 92. 234 308 IHC; tested species: mouse

Retinal response to systemic inflammation differs between sexes and neurons.
Rodríguez-Ramírez KT, Norte-Muñoz M, Lucas-Ruiz F, Gallego-Ortega A, Calzaferri F, García-Bernal D, Martínez CM, Galindo-Romero C, de Los Ríos C, Vidal-Sanz M, Agudo-Barriuso M, et al.
Frontiers in immunology (2024) 15: 1340013. 234 308 IHC; tested species: mouse

Receptor-interacting protein kinase 2 (RIPK2) profoundly contributes to post-stroke neuroinflammation and behavioral deficits with microglia as unique perpetrators.
Larochelle J, Tishko RJ, Yang C, Ge Y, Phan LT, Gunraj RE, Stansbury SM, Liu L, Mohamadzadeh M, Khoshbouei H, Candelario-Jalil E, et al.
Journal of neuroinflammation (2023) 201: 221. 234 308 IHC; tested species: mouse

Human microglial state dynamics in Alzheimer's disease progression.
Sun N, Victor MB, Park YP, Xiong X, Scannail AN, Leary N, Prosper S, Viswanathan S, Luna X, Boix CA, James BT, et al.
Cell (2023) 18620: 4386-4403.e29. 234 308 IHC; tested species: human

Microglial Nogo delays recovery following traumatic brain injury in mice.
Glotfelty EJ, Hsueh SC, Claybourne Q, Bedolla A, Kopp KO, Wallace T, Zheng B, Luo Y, Karlsson TE, McDevitt RA, Olson L, et al.
Glia (2023) 7110: 2473-2494. 234 308 IHC; tested species: mouse

Trem2 expression in microglia is required to maintain normal neuronal bioenergetics during development.
Tagliatti E, Desiato G, Mancinelli S, Bizzotto M, Gagliani MC, Faggiani E, Hernández-Soto R, Cugurra A, Poliseno P, Miotto M, Argüello RJ, et al.
Immunity (2023) : . 234 308 IHC; tested species: mouse

Chronic exposure to a synthetic cannabinoid improves cognition and increases locomotor activity in Tg4-42 Alzheimer's disease mice.
Ott FW, Sichler ME, Bouter C, Enayati M, Wiltfang J, Bayer TA, Beindorff N, Löw MJ, Bouter Y
Journal of Alzheimer's disease reports (2025) 9: 25424823241306770. 234 308 IHC-P; tested species: mouse

Reliable detection of RNA in hippocampus sections of mice by FISH up to a post-mortem delay of 24 h.
Seiffer S, Brendler J, Schulz A, Ricken A
Histochemistry and cell biology (2024) : . 234 308 IHC-P; tested species: mouse

Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 234 308 IHC-P; tested species: mouse

Cat. No.: 234 308

Amount: 50 µg

Price: $430.00

References
WB (1)
ICC (4)
IHC (19)
IHC-P (3)

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 234 308 ICC; tested species: mouse

ICC

IHC

IHC-P

Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).

1 ) Köhler C et al., Cell Tissue Res. (2007)
2 ) Deininger MH et al., Acta Neuropathol. (2000)
3 ) Gehrmann J et al., Brain Res. Brain Res. Rev. (1995)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

IBA1 antibody - 234 308

References for IBA1 - 234 308