Synaptic Systems - GABA-A receptor alpha1

Cat. No.: 224 211

Amount: 100 µg

Price: $415.00

Cat. No. 224 211	100 µg purified IgM, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 gallery IHC-P: not tested yet EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	53D3
Subtype	IgM (κ light chain)
Immunogen	Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity	Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.
Matching control protein/peptide	224-2P
Remarks	ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents.
Data sheet	224_211.pdf

References for GABA-A receptor alpha1 - 224 211

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism.
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D, et al.
Nature communications (2020) 111: 2140. 224 211 ICC; tested species: mouse

Brefeldin A-inhibited guanine nucleotide exchange protein 3 is localized in lysosomes and regulates GABA signaling in hippocampal neurons.
Liu T, Li H, Hong W, Han W
Journal of neurochemistry (2016) 1395: 748-756. 224 211 ICC

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 211 EM

Cat. No.: 224 211

Amount: 100 µg

Price: $415.00

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 211 EM

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Laurie DJ et al., J. Neurosci. (1992)
2 ) Laurie DJ et al., J. Neurosci. (1992)
3 ) Wisden W et al., J. Neurosci. (1992)
4 ) Lüddens H et al., Nature (1990)
5 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

GABA-A receptor alpha1 antibody extracellular - 224 211

References for GABA-A receptor alpha1 - 224 211