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GABA-A receptor alpha1 - 224 211

Receptors mediating inhibitory neurotransmission
Monoclonal mouse purified IgM
Cat. No.: 224 211
Amount: 100 µg
Price: 415.00 €
Cat. No. 224 211 100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes
Clone 53D3
Subtype IgM (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Epitop Epitop: AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1.
Matching control protein/peptide 224-2P
Remarks

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: This antibody requires mild fixation.

Data sheet 224_211.pdf

References for GABA-A receptor alpha1 - 224 211

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB
Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism.
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D, et al.
Nature communications (2020) 111: 2140. 224 211 ICC; tested species: mouse
Brefeldin A-inhibited guanine nucleotide exchange protein 3 is localized in lysosomes and regulates GABA signaling in hippocampal neurons.
Liu T, Li H, Hong W, Han W
Journal of neurochemistry (2016) 1395: 748-756. 224 211 ICC
Cat. No.: 224 211
Quantity: 100 µg
Price: 415.00 €
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB
Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism.
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D, et al.
Nature communications (2020) 111: 2140. 224 211 ICC; tested species: mouse
Brefeldin A-inhibited guanine nucleotide exchange protein 3 is localized in lysosomes and regulates GABA signaling in hippocampal neurons.
Liu T, Li H, Hong W, Han W
Journal of neurochemistry (2016) 1395: 748-756. 224 211 ICC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.