|Cat. No. 224 208AT488||
50 µg purified recombinant IgG, lyophilized, fluorescence-labeled with ATTO® 488.
For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from
ATTO®488, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: N/A
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 (see remarks) gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)|
Reacts with: mouse (P62812), rat (P62813).
Other species not tested yet.
|Matching control protein/peptide||224-2P|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 53D3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.