|Cat. No. 224 208||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)|
Epitop: AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
|Specificity||Specific for GABA-A receptor α1|
|Matching control protein/peptide||224-2P|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 53D3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.