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Home > Products > 224 205

GABA-A receptor alpha1 antibody extracellular - 224 205 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig polyclonal purified antibody
Cat. No.: 224 205
Amount: 50 µg
Price: $465.00
Cat. No. 224 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity K.O. validated PubMed: 39261733
Matching control protein/peptide 224-2P
Remarks

WB:  GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
Data sheet 224_205.pdf

References for GABA-A receptor alpha1 - 224 205

A brain-to-gut signal controls intestinal fat absorption.
Lyu Q, Xue W, Liu R, Ma Q, Kasaragod VB, Sun S, Li Q, Chen Y, Yuan M, Yang Y, Zhang B, et al.
Nature (2024) : . 224 205 WB, IHC; KO verified; tested species: mouse
Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins.
Kasaragod VB, Hausrat TJ, Schaefer N, Kuhn M, Christensen NR, Tessmer I, Maric HM, Madsen KL, Sotriffer C, Villmann C, Kneussel M, et al.
Neuron (2019) 1014: 673-689.e11. 224 205 WB; tested species: mouse
Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.
Roesler MK, Lombino FL, Freitag S, Schweizer M, Hermans-Borgmeyer I, Schwarz JR, Kneussel M, Wagner W
Frontiers in cellular neuroscience (2019) 13: 330. 224 205 WB; tested species: mouse
Cat. No.: 224 205
Amount: 50 µg
Price: $465.00
WB
ICC
IHC
IHC
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