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GABA-A receptor alpha1 - 224 205

Receptors mediating inhibitory neurotransmission
Polyclonal Guinea pig purified antibody
Cat. No.: 224 205
Amount: 50 µg
Price: 450.00 €
Cat. No. 224 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1. K.O.
Matching control protein/peptide 224-2P
Remarks

WB:  GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 224_205.pdf

References for GABA-A receptor alpha1 - 224 205

Tonic GABAergic Inhibition Is Essential for Nerve Injury-Induced Afferent Remodeling in the Somatosensory Thalamus and Ectopic Sensations.
Nagumo Y, Ueta Y, Nakayama H, Osaki H, Takeuchi Y, Uesaka N, Kano M, Miyata M
Cell reports (2020) 3112: 107797. 224 205 IHC; tested species: rat
Early-life adversity selectively impairs α2-GABAA receptor expression in the mouse nucleus accumbens and influences the behavioral effects of cocaine.
Mitchell SJ, Maguire EP, Cunningham L, Gunn BG, Linke M, Zechner U, Dixon CI, King SL, Stephens DN, Swinny JD, Belelli D, et al.
Neuropharmacology (2018) : . 224 205 IHC; tested species: mouse
Cat. No.: 224 205
Quantity: 50 µg
Price: 450.00 €
Tonic GABAergic Inhibition Is Essential for Nerve Injury-Induced Afferent Remodeling in the Somatosensory Thalamus and Ectopic Sensations.
Nagumo Y, Ueta Y, Nakayama H, Osaki H, Takeuchi Y, Uesaka N, Kano M, Miyata M
Cell reports (2020) 3112: 107797. 224 205 IHC; tested species: rat
Early-life adversity selectively impairs α2-GABAA receptor expression in the mouse nucleus accumbens and influences the behavioral effects of cocaine.
Mitchell SJ, Maguire EP, Cunningham L, Gunn BG, Linke M, Zechner U, Dixon CI, King SL, Stephens DN, Swinny JD, Belelli D, et al.
Neuropharmacology (2018) : . 224 205 IHC; tested species: mouse
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.