Synaptic Systems - GABA-A receptor alpha1

Cat. No.: 224 203

Amount: 50 µg

Price: $385.00

Cat. No. 224 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 (see remarks) gallery IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery IHC-P: not tested yet
Immunogen	Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity	Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.
Specificity	K.O. validated PubMed: 25080596
Matching control protein/peptide	224-2P
Remarks	WB: GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Data sheet	224_203.pdf

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors.
Whittsette AL, Wang YJ, Mu TW
iScience (2022) 258: 104754. 224 203 WB, IP, UPTAKE; tested species: rat

Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 224 203 WB; tested species: mouse

Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat

Network Neuromodulation of Opioid and GABAergic Receptors Following a Combination of "Juvenile" and "Adult Stress" in Rats.
Horovitz O, Ardi Z, Ashkenazi SK, Ritov G, Anunu R, Richter-Levin G
International journal of molecular sciences (2020) 2115: . 224 203 WB; tested species: rat

The role of the GABAA receptor Alpha 1 subunit in the ventral hippocampus in stress resilience.
Ardi Z, Richter-Levin A, Xu L, Cao X, Volkmer H, Stork O, Richter-Levin G
Scientific reports (2019) 91: 13513. 224 203 WB; tested species: rat

Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions.
Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, et al.
eLife (2019) 8: . 224 203 WB, IHC; tested species: mouse

Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse

Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 203 WB

Cat. No.: 224 203

Amount: 50 µg

Price: $385.00

ICC

IHC

Background

Protocols

GABA-A receptor alpha1 antibody extracellular - 224 203 K.O.