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GABA-A receptor alpha1 - 224 203

Receptors mediating inhibitory neurotransmission
Polyclonal rabbit purified antibody
Cat. No.: 224 203
Amount: 50 µg
Price: 370.00 €
Cat. No. 224 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1. K.O. PubMed: 25080596
Matching control protein/peptide 224-2P
Remarks

WB:  GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Data sheet 224_203.pdf

References for GABA-A receptor alpha1 - 224 203

Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 224 203 WB; tested species: mouse
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
The role of the GABAA receptor Alpha 1 subunit in the ventral hippocampus in stress resilience.
Ardi Z, Richter-Levin A, Xu L, Cao X, Volkmer H, Stork O, Richter-Levin G
Scientific reports (2019) 91: 13513. 224 203 WB; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 203 WB
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 203 ICC; tested species: rat
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 203 ICC; tested species: mouse
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 203 IHC; tested species: rat
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 224 203 IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 203 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 203 IHC; tested species: mouse
Structural and functional characterization of dendritic arbors and GABAergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala.
Klenowski PM, Fogarty MJ, Belmer A, Noakes PG, Bellingham MC, Bartlett SE
Journal of neurophysiology (2015) 1142: 942-57. 224 203 IHC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 224 203 IHC; tested species: mouse
Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.
Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3428: 9418-31. 224 203 IHC; tested species: mouse
Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon.
Seifi M, Brown JF, Mills J, Bhandari P, Belelli D, Lambert JJ, Rudolph U, Swinny JD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10361-78. 224 203 IHC; KO verified; tested species: mouse
Cat. No.: 224 203
Quantity: 50 µg
Price: 370.00 €
Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 224 203 WB; tested species: mouse
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
The role of the GABAA receptor Alpha 1 subunit in the ventral hippocampus in stress resilience.
Ardi Z, Richter-Levin A, Xu L, Cao X, Volkmer H, Stork O, Richter-Levin G
Scientific reports (2019) 91: 13513. 224 203 WB; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder.
Ardi Z, Albrecht A, Richter-Levin A, Saha R, Richter-Levin G
Neurobiology of disease (2016) 88: 139-47. 224 203 WB
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 203 ICC; tested species: rat
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 203 ICC; tested species: mouse
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one (2012) 78: e42959. 224 203 WB, ICC
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 203 IHC; tested species: rat
Enriched environment ameliorates memory impairments in rats after postsurgery sleep deprivation.
Gao J, Yang C, Li D, Zhao L, Wang H
Journal of chemical neuroanatomy (2020) : 101850. 224 203 WB, IHC; tested species: rat
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 224 203 IHC; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 224 203 WB, IHC; tested species: mouse
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 203 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 224 203 IHC; tested species: mouse
Structural and functional characterization of dendritic arbors and GABAergic synaptic inputs on interneurons and principal cells in the rat basolateral amygdala.
Klenowski PM, Fogarty MJ, Belmer A, Noakes PG, Bellingham MC, Bartlett SE
Journal of neurophysiology (2015) 1142: 942-57. 224 203 IHC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 224 203 IHC; tested species: mouse
Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei.
Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3428: 9418-31. 224 203 IHC; tested species: mouse
Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon.
Seifi M, Brown JF, Mills J, Bhandari P, Belelli D, Lambert JJ, Rudolph U, Swinny JD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10361-78. 224 203 IHC; KO verified; tested species: mouse
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.