|Cat. No. 224 203||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 (see remarks) gallery
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)|
Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
|Specificity||Specific for GABA-A receptor α1. K.O. PubMed: 25080596|
|Matching control protein/peptide||224-2P|
WB: GABA-A receptor alpha1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondaryreagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.