MAP2 antibody - 188 011BT

MAP2 is a major microtubule associated protein in neurons

Mouse monoclonal purified IgG

Cat. No.: 188 011BT

Amount: 100 µg

Price: $475.00

Cat. No. 188 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 100 up to 1 : 200 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	198A5
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Epitop	AA 82 to 96 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity	Reacts with: human (P11137), rat (P15146), mouse (P20357). No signal: zebrafish. Other species not tested yet.
Specificity	Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide	188-0P
Data sheet	188_011bt.pdf

References for MAP2 - 188 011BT

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 188 011BT IHC; tested species: mouse

Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 011BT IHC

Cat. No.: 188 011BT

Amount: 100 µg

Price: $475.00

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP2 have been described so far.

1 ) Dehmelt L et al., Genome Biol. (2005)
2 ) Al-Bassam J et al., J. Cell Biol. (2002)
3 ) Di Stefano G et al., J. Histochem. Cytochem. (2001)
4 ) Chauhan N et al., Neurochem. Res. (1997)
5 ) Sánchez C et al., Biochem. J. (1995)
6 ) Izant JG et al., Proc. Natl. Acad. Sci. U.S.A. (1980)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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