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MAP 2 - 188 011

A major microtubule associated protein
Monoclonal mouse purified IgG
Cat. No.: 188 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 188 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 200 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Clone 198A5
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 2 to 314 from human MAP2-4 hu (UniProt Id: P11137-4)
Epitop Epitop: AA 82 to 96 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity Reacts with: human (P11137), rat (P15146), mouse (P20357).
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Remarks

WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 188_011.pdf

References for MAP 2 - 188 011

Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia.
Liu Y, Xue X, Zhang H, Che X, Luo J, Wang P, Xu J, Xing Z, Yuan L, Liu Y, Fu X, et al.
Autophagy (2018) : . 188 011 WB; tested species: rat
Genetic inactivation of mTORC1 or mTORC2 in neurons reveals distinct functions in glutamatergic synaptic transmission.
McCabe MP, Cullen ER, Barrows CM, Shore AN, Tooke KI, Laprade KA, Stafford JM, Weston MC
eLife (2020) 9: . 188 011 ICC; tested species: mouse
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 011 ICC; tested species: human
Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines.
Mikhaylova M, Bär J, van Bommel B, Schätzle P, YuanXiang P, Raman R, Hradsky J, Konietzny A, Loktionov EY, Reddy PP, Lopez-Rojas J, et al.
Neuron (2018) 975: 1110-1125.e14. 188 011 ICC; tested species: mouse
Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant.
Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl AR, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 29: 60-63. 188 011 ICC; tested species: human
Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture.
Moretti D, DiFrancesco ML, Sharma PP, Dante S, Albisetti E, Monticelli M, Bertacco R, Petti D, Baldelli P, Benfenati F
Frontiers in neuroscience (2018) 12: 909. 188 011 ICC; tested species: rat
PTEN Loss Increases the Connectivity of Fast Synaptic Motifs and Functional Connectivity in a Developing Hippocampal Network.
Barrows CM, McCabe MP, Chen H, Swann JW, Weston MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3736: 8595-8611. 188 011 ICC; tested species: mouse
BMP7-induced dendritic growth in sympathetic neurons requires p75(NTR) signaling.
Courter LA, Shaffo FC, Ghogha A, Parrish DJ, Lorentz CU, Habecker BA, Lein PJ
Developmental neurobiology (2016) 769: 1003-13. 188 011 ICC
Brain extracellular matrix retains connectivity in neuronal networks.
Bikbaev A, Frischknecht R, Heine M
Scientific reports (2015) 5: 14527. 188 011 ICC
Combinatorial hedgehog and mitogen signaling promotes the in vitro expansion but not retinal differentiation potential of retinal progenitor cells.
Ringuette R, Wang Y, Atkins M, Mears AJ, Yan K, Wallace VA
Investigative ophthalmology & visual science (2014) 551: 43-54. 188 011 ICC; tested species: mouse
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 188 011 ICC; tested species: mouse
A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion and in vitro cell culturing.
Tonna N, Bianco F, Matteoli M, Cagnoli C, Antonucci F, Manfredi A, Mauro N, Ranucci E, Ferruti P
Science and technology of advanced materials (2014) 154: 045007. 188 011 ICC
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 188 011 ICC
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 188 011 IHC; tested species: mouse
Genetically Induced Retrograde Amnesia of Associative Memories After Neuroplastin Ablation.
Bhattacharya S, Herrera-Molina R, Sabanov V, Ahmed T, Iscru E, Stöber F, Richter K, Fischer KD, Angenstein F, Goldschmidt J, Beesley PW, et al.
Biological psychiatry (2017) 812: 124-135. 188 011 IHC
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 011 IHC
Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.
Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, et al.
Stem cell research & therapy (2015) 6: 92. 188 011 IHC; tested species: mouse
Cat. No.: 188 011
Quantity: 100 µg
Price: 415.00 €
Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia.
Liu Y, Xue X, Zhang H, Che X, Luo J, Wang P, Xu J, Xing Z, Yuan L, Liu Y, Fu X, et al.
Autophagy (2018) : . 188 011 WB; tested species: rat
Genetic inactivation of mTORC1 or mTORC2 in neurons reveals distinct functions in glutamatergic synaptic transmission.
McCabe MP, Cullen ER, Barrows CM, Shore AN, Tooke KI, Laprade KA, Stafford JM, Weston MC
eLife (2020) 9: . 188 011 ICC; tested species: mouse
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 011 ICC; tested species: human
Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines.
Mikhaylova M, Bär J, van Bommel B, Schätzle P, YuanXiang P, Raman R, Hradsky J, Konietzny A, Loktionov EY, Reddy PP, Lopez-Rojas J, et al.
Neuron (2018) 975: 1110-1125.e14. 188 011 ICC; tested species: mouse
Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant.
Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl AR, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 29: 60-63. 188 011 ICC; tested species: human
Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture.
Moretti D, DiFrancesco ML, Sharma PP, Dante S, Albisetti E, Monticelli M, Bertacco R, Petti D, Baldelli P, Benfenati F
Frontiers in neuroscience (2018) 12: 909. 188 011 ICC; tested species: rat
PTEN Loss Increases the Connectivity of Fast Synaptic Motifs and Functional Connectivity in a Developing Hippocampal Network.
Barrows CM, McCabe MP, Chen H, Swann JW, Weston MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3736: 8595-8611. 188 011 ICC; tested species: mouse
BMP7-induced dendritic growth in sympathetic neurons requires p75(NTR) signaling.
Courter LA, Shaffo FC, Ghogha A, Parrish DJ, Lorentz CU, Habecker BA, Lein PJ
Developmental neurobiology (2016) 769: 1003-13. 188 011 ICC
Brain extracellular matrix retains connectivity in neuronal networks.
Bikbaev A, Frischknecht R, Heine M
Scientific reports (2015) 5: 14527. 188 011 ICC
Combinatorial hedgehog and mitogen signaling promotes the in vitro expansion but not retinal differentiation potential of retinal progenitor cells.
Ringuette R, Wang Y, Atkins M, Mears AJ, Yan K, Wallace VA
Investigative ophthalmology & visual science (2014) 551: 43-54. 188 011 ICC; tested species: mouse
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 188 011 ICC; tested species: mouse
A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion and in vitro cell culturing.
Tonna N, Bianco F, Matteoli M, Cagnoli C, Antonucci F, Manfredi A, Mauro N, Ranucci E, Ferruti P
Science and technology of advanced materials (2014) 154: 045007. 188 011 ICC
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 188 011 ICC
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 188 011 IHC; tested species: mouse
Genetically Induced Retrograde Amnesia of Associative Memories After Neuroplastin Ablation.
Bhattacharya S, Herrera-Molina R, Sabanov V, Ahmed T, Iscru E, Stöber F, Richter K, Fischer KD, Angenstein F, Goldschmidt J, Beesley PW, et al.
Biological psychiatry (2017) 812: 124-135. 188 011 IHC
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 011 IHC
Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.
Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, et al.
Stem cell research & therapy (2015) 6: 92. 188 011 IHC; tested species: mouse
Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.