Synaptic Systems

Cat. No.: 188 011

Amount: 100 µg

Price: $425.00

Cat. No. 188 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 100 up to 1 : 200 gallery IHC-P: 1 : 500 gallery DNA-PAINT: yes (see remarks)
Clone	198A5
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human Map2 (UniProt Id: P11137-4)
Epitop	AA 82 to 96 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity	Reacts with: human (P11137), rat (P15146), mouse (P20357). No signal: zebrafish. Other species not tested yet.
Specificity	Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide	188-0P
Remarks	WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	188_011.pdf

References for MAP2 - 188 011

WB (1)
ICC (22)
IHC (8)
IHC-P (1)
DNA-PAINT (1)

Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia.
Liu Y, Xue X, Zhang H, Che X, Luo J, Wang P, Xu J, Xing Z, Yuan L, Liu Y, Fu X, et al.
Autophagy (2018) : . 188 011 WB; tested species: rat

TRPM2 and CaMKII Signaling Drives Excessive GABAergic Synaptic Inhibition Following Ischemia.
Burch AM, Garcia JD, O'Leary H, Haas A, Orfila JE, Tiemeier E, Chalmers N, Smith KR, Quillinan N, Herson PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4419: . 188 011 ICC; tested species: mouse

Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 188 011 ICC; tested species: rat

High-Content Screening of Synaptic Density Modulators in Primary Neuronal Cultures.
Coulon A, Siedlecki-Wullich D, Najdek C, Gelle C, Ayral AM, Demiautte F, Lambert E, Vandeputte A, Brodin P, Mendes T, Lambert JC, et al.
Current protocols (2023) 310: e904. 188 011 ICC; tested species: rat

Aminoprocalcitonin protects against hippocampal neuronal death via preserving oxidative phosphorylation in refractory status epilepticus.
Song C, Zhao J, Hao J, Mi D, Zhang J, Liu Y, Wu S, Gao F, Jiang W
Cell death discovery (2023) 91: 144. 188 011 ICC; tested species: rat

Disruption of mTORC1 rescues neuronal overgrowth and synapse function dysregulated by Pten loss.
Tariq K, Cullen E, Getz SA, Conching AKS, Goyette AR, Prina ML, Wang W, Li M, Weston MC, Luikart BW
Cell reports (2022) 415: 111574. 188 011 ICC; tested species: mouse

Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization.
Halff EF, Hannan S, Kwanthongdee J, Lesept F, Smart TG, Kittler JT
Science signaling (2022) 15739: eabg2505. 188 011 ICC; tested species: rat

Muskelin regulates actin-dependent synaptic changes and intrinsic brain activity relevant to behavioral and cognitive processes.
Muhia M, YuanXiang P, Sedlacik J, Schwarz JR, Heisler FF, Gromova KV, Thies E, Breiden P, Pechmann Y, Kreutz MR, Kneussel M, et al.
Communications biology (2022) 51: 589. 188 011 ICC; tested species: human

FEZ1 forms complexes with CRMP1 and DCC to regulate axon and dendrite development.
Chua JY, Ng SJ, Yagensky O, Wanker EE, Chua JJE
eNeuro (2021) : . 188 011 ICC; tested species: rat

ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 188 011 ICC; tested species: mouse

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 188 011 ICC; tested species: human

Genetic inactivation of mTORC1 or mTORC2 in neurons reveals distinct functions in glutamatergic synaptic transmission.
McCabe MP, Cullen ER, Barrows CM, Shore AN, Tooke KI, Laprade KA, Stafford JM, Weston MC
eLife (2020) 9: . 188 011 ICC; tested species: mouse

Pyk2 overexpression in postsynaptic neurons blocks amyloid β1-42-induced synaptotoxicity in microfluidic co-cultures.
Kilinc D, Vreulx AC, Mendes T, Flaig A, Marques-Coelho D, Verschoore M, Demiautte F, Amouyel P, , Eysert F, Dourlen P, et al.
Brain communications (2020) 22: fcaa139. 188 011 ICC; tested species: rat

Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture.
Moretti D, DiFrancesco ML, Sharma PP, Dante S, Albisetti E, Monticelli M, Bertacco R, Petti D, Baldelli P, Benfenati F
Frontiers in neuroscience (2018) 12: 909. 188 011 ICC; tested species: rat

Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant.
Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl AR, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research (2018) 29: 60-63. 188 011 ICC; tested species: human

Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines.
Mikhaylova M, Bär J, van Bommel B, Schätzle P, YuanXiang P, Raman R, Hradsky J, Konietzny A, Loktionov EY, Reddy PP, Lopez-Rojas J, et al.
Neuron (2018) 975: 1110-1125.e14. 188 011 ICC; tested species: mouse

PTEN Loss Increases the Connectivity of Fast Synaptic Motifs and Functional Connectivity in a Developing Hippocampal Network.
Barrows CM, McCabe MP, Chen H, Swann JW, Weston MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3736: 8595-8611. 188 011 ICC; tested species: mouse

BMP7-induced dendritic growth in sympathetic neurons requires p75(NTR) signaling.
Courter LA, Shaffo FC, Ghogha A, Parrish DJ, Lorentz CU, Habecker BA, Lein PJ
Developmental neurobiology (2016) 769: 1003-13. 188 011 ICC

Brain extracellular matrix retains connectivity in neuronal networks.
Bikbaev A, Frischknecht R, Heine M
Scientific reports (2015) 5: 14527. 188 011 ICC

A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion and in vitro cell culturing.
Tonna N, Bianco F, Matteoli M, Cagnoli C, Antonucci F, Manfredi A, Mauro N, Ranucci E, Ferruti P
Science and technology of advanced materials (2014) 154: 045007. 188 011 ICC

Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 188 011 ICC; tested species: mouse

Combinatorial hedgehog and mitogen signaling promotes the in vitro expansion but not retinal differentiation potential of retinal progenitor cells.
Ringuette R, Wang Y, Atkins M, Mears AJ, Yan K, Wallace VA
Investigative ophthalmology & visual science (2014) 551: 43-54. 188 011 ICC; tested species: mouse

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 188 011 ICC

The role of α-tubulin tyrosination in controlling the structure and function of hippocampal neurons.
Hosseini S, van Ham M, Erck C, Korte M, Michaelsen-Preusse K
Frontiers in molecular neuroscience (2022) 15: 931859. 188 011 IHC; tested species: mouse

Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition.
Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P
Neuron (2022) 11013: 2094-2109.e10. 188 011 IHC; tested species: mouse

mTORC2 inhibition improves morphological effects of PTEN loss, but does not correct synaptic dysfunction or prevent seizures.
Cullen ER, Tariq K, Shore AN, Luikart BW, Weston MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 188 011 IHC; tested species: mouse

Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 188 011 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 188 011 IHC; tested species: mouse

Genetically Induced Retrograde Amnesia of Associative Memories After Neuroplastin Ablation.
Bhattacharya S, Herrera-Molina R, Sabanov V, Ahmed T, Iscru E, Stöber F, Richter K, Fischer KD, Angenstein F, Goldschmidt J, Beesley PW, et al.
Biological psychiatry (2017) 812: 124-135. 188 011 IHC

Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 011 IHC

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.
Zhou F, Gao S, Wang L, Sun C, Chen L, Yuan P, Zhao H, Yi Y, Qin Y, Dong Z, Cao L, et al.
Stem cell research & therapy (2015) 6: 92. 188 011 IHC; tested species: mouse

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. 188 011 IHC-P; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 188 011 DNA-PAINT; tested species: rat

Cat. No.: 188 011

Amount: 100 µg

Price: $425.00

References
WB (1)
ICC (22)
IHC (8)
IHC-P (1)
DNA-PAINT (1)

Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 188 011 ICC; tested species: rat

FEZ1 forms complexes with CRMP1 and DCC to regulate axon and dendrite development.
Chua JY, Ng SJ, Yagensky O, Wanker EE, Chua JJE
eNeuro (2021) : . 188 011 ICC; tested species: rat

ZnT1 is a neuronal Zn2+/Ca2+ exchanger.
Gottesman N, Asraf H, Bogdanovic M, Sekler I, Tzounopoulos T, Aizenman E, Hershfinkel M
Cell calcium (2021) 101: 102505. 188 011 ICC; tested species: mouse

Brain extracellular matrix retains connectivity in neuronal networks.
Bikbaev A, Frischknecht R, Heine M
Scientific reports (2015) 5: 14527. 188 011 ICC

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP2 have been described so far.

1 ) Dehmelt L et al., Genome Biol. (2005)
2 ) Al-Bassam J et al., J. Cell Biol. (2002)
3 ) Di Stefano G et al., J. Histochem. Cytochem. (2001)
4 ) Chauhan N et al., Neurochem. Res. (1997)
5 ) Sánchez C et al., Biochem. J. (1995)
6 ) Izant JG et al., Proc. Natl. Acad. Sci. U.S.A. (1980)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

MAP2 antibody - 188 011

References for MAP2 - 188 011