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MAP 2 - 188 006

A major microtubule associated protein
Polyclonal chicken purified antibody
Cat. No.: 188 006
Amount: 50 µg
Price: $395.00
Cat. No. 188 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 314 from human MAP2-4 hu (UniProt Id: P11137-4)
Reactivity Reacts with: rat (P15146), mouse (P20357).
Other species not tested yet.
Specificity Specific for MAP 2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Remarks

WB: Due to its large size, MAP 2 requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 188_006.pdf

References for MAP 2 - 188 006

High-Content Screening for Protein-Protein Interaction Modulators Using Proximity Ligation Assay in Primary Neurons.
Mendes T, Herledan A, Leroux F, Deprez B, Lambert JC, Kilinc D
Current protocols in cell biology (2020) 861: e100. 188 006 ICC; tested species: rat
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 188 006 ICC; tested species: mouse
High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity.
Verschuuren M, Verstraelen P, García-Díaz Barriga G, Cilissen I, Coninx E, Verslegers M, Larsen PH, Nuydens R, De Vos WH
Acta neuropathologica communications (2019) 71: 6. 188 006 ICC; tested species: mouse
BIN1 recovers tauopathy-induced long-term memory deficits in mice and interacts with Tau through Thr348 phosphorylation.
Sartori M, Mendes T, Desai S, Lasorsa A, Herledan A, Malmanche N, Mäkinen P, Marttinen M, Malki I, Chapuis J, Flaig A, et al.
Acta neuropathologica (2019) 1384: 631-652. 188 006 ICC; tested species: rat
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 188 006 IHC; tested species: rat
Analysis of Synapses in Cerebral Organoids.
Yakoub AM, Sadek M
Cell transplantation (2019) : 963689718822811. 188 006 IHC; tested species: human
Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment.
Alshammari MA, Alshammari TK, Laezza F
Frontiers in cellular neuroscience (2016) 10: 5. 188 006 IHC
Cat. No.: 188 006
Quantity: 50 µg
Price: $395.00
High-Content Screening for Protein-Protein Interaction Modulators Using Proximity Ligation Assay in Primary Neurons.
Mendes T, Herledan A, Leroux F, Deprez B, Lambert JC, Kilinc D
Current protocols in cell biology (2020) 861: e100. 188 006 ICC; tested species: rat
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 188 006 ICC; tested species: mouse
High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity.
Verschuuren M, Verstraelen P, García-Díaz Barriga G, Cilissen I, Coninx E, Verslegers M, Larsen PH, Nuydens R, De Vos WH
Acta neuropathologica communications (2019) 71: 6. 188 006 ICC; tested species: mouse
BIN1 recovers tauopathy-induced long-term memory deficits in mice and interacts with Tau through Thr348 phosphorylation.
Sartori M, Mendes T, Desai S, Lasorsa A, Herledan A, Malmanche N, Mäkinen P, Marttinen M, Malki I, Chapuis J, Flaig A, et al.
Acta neuropathologica (2019) 1384: 631-652. 188 006 ICC; tested species: rat
Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.
Klevanski M, Herrmannsdoerfer F, Sass S, Venkataramani V, Heilemann M, Kuner T
Nature communications (2020) 111: 1552. 188 006 IHC; tested species: rat
Analysis of Synapses in Cerebral Organoids.
Yakoub AM, Sadek M
Cell transplantation (2019) : 963689718822811. 188 006 IHC; tested species: human
Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment.
Alshammari MA, Alshammari TK, Laezza F
Frontiers in cellular neuroscience (2016) 10: 5. 188 006 IHC
Background

There are two major classes of heat stable microtubule associated proteins (MAPs): MAP 2, and tau. Both protein classes are involved in the regulation of microtubule polymerization in cells.
Four differentially regulated isoforms of MAP 2 have been described so far.