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GluA - 182 403

A member of a receptor group involved in rapid excitatory neurotransmission in the mammalian CNS
Polyclonal rabbit purified antibody
Cat. No.: 182 403
Amount: 50 µg
Price: 370.00 €
Cat. No. 182 403 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 742 to 798 from rat GluA1 (UniProt Id: P19490)
Reactivity Reacts with: human (P42261, P42262, P42263, P48058), rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8).
Other species not tested yet.
Specificity Raised against GluA 1 but, due to sequence homology, likely to crossreact with GluA 2, 3, and 4.
Remarks

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

The antibody is also suitable for EM based replica technique.

Data sheet 182_403.pdf

References for GluA - 182 403

KV10.1 opposes activity-dependent increase in Ca2+ influx into the presynaptic terminal of the parallel fibre - Purkinje cell synapse.
Mortensen LS, Schmidt H, Farsi Z, Barrantes-Freer A, Rubio ME, Ufartes R, Eilers J, Sakaba T, Stühmer W, Pardo LA
The Journal of physiology (2014) : . 182 403 WB
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 182 403 WB
Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 403 WB
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 403 EM; tested species: mouse
Objective quantification of nanoscale protein distributions.
Szoboszlay M, Kirizs T, Nusser Z
Scientific reports (2017) 71: 15240. 182 403 EM; tested species: mouse
Cat. No.: 182 403
Quantity: 50 µg
Price: 370.00 €
KV10.1 opposes activity-dependent increase in Ca2+ influx into the presynaptic terminal of the parallel fibre - Purkinje cell synapse.
Mortensen LS, Schmidt H, Farsi Z, Barrantes-Freer A, Rubio ME, Ufartes R, Eilers J, Sakaba T, Stühmer W, Pardo LA
The Journal of physiology (2014) : . 182 403 WB
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 182 403 WB
Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 403 WB
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 403 EM; tested species: mouse
Objective quantification of nanoscale protein distributions.
Szoboszlay M, Kirizs T, Nusser Z
Scientific reports (2017) 71: 15240. 182 403 EM; tested species: mouse
Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.