|Cat. No. 182 403||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC-P/FFPE: not tested yet
EM: yes gallery
|Immunogen||Recombinant protein corresponding to AA 742 to 798 from rat GluA1 (UniProt Id: P19490)|
Reacts with: human (P42261, P42262, P42263, P48058), rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8).
Other species not tested yet.
|Specificity||Raised against GluA 1 but, due to sequence homology, likely to crossreact with GluA 2, 3, and 4.|
IHC: This antibody requires antigen retrieval with pepsin according to:
Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.