Synaptic Systems

Cat. No.: 182 403

Amount: 50 µg

Price: $375.00

Cat. No. 182 403	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 742 to 798 from rat GluA1 (UniProt Id: P19490)
Reactivity	Reacts with: human (P42261, P42262, P42263, P48058), rat (P19490, P19491, P19492, P19493), mouse (P23818, P23819, Q9Z2W9, Q9Z2W8). Other species not tested yet.
Specificity	Raised against GluA 1 but, due to sequence homology, likely to crossreact with GluA 2, 3, and 4.
Remarks	IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). The antibody is also suitable for EM based replica technique.
Data sheet	182_403.pdf

References for GluA - 182 403

KV10.1 opposes activity-dependent increase in Ca2+ influx into the presynaptic terminal of the parallel fibre - Purkinje cell synapse.
Mortensen LS, Schmidt H, Farsi Z, Barrantes-Freer A, Rubio ME, Ufartes R, Eilers J, Sakaba T, Stühmer W, Pardo LA
The Journal of physiology (2014) : . 182 403 WB

Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 182 403 WB

Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Schmeisser MJ, Ey E, Wegener S, Bockmann J, Stempel AV, Kuebler A, Janssen AL, Udvardi PT, Shiban E, Spilker C, Balschun D, et al.
Nature (2012) 4867402: 256-60. 182 403 WB

A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors.
Boudkkazi S, Schwenk J, Nakaya N, Brechet A, Kollewe A, Harada H, Bildl W, Kulik A, Dong L, Sultana A, Zolles G, et al.
Neuron (2023) 11116: 2544-2556.e9. 182 403 EM; tested species: mouse

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
Schwenk J, Boudkkazi S, Kocylowski MK, Brechet A, Zolles G, Bus T, Costa K, Kollewe A, Jordan J, Bank J, Bildl W, et al.
Neuron (2019) : . 182 403 EM; tested species: mouse

Objective quantification of nanoscale protein distributions.
Szoboszlay M, Kirizs T, Nusser Z
Scientific reports (2017) 71: 15240. 182 403 EM; tested species: mouse

Cat. No.: 182 403

Amount: 50 µg

Price: $375.00

Objective quantification of nanoscale protein distributions.
Szoboszlay M, Kirizs T, Nusser Z
Scientific reports (2017) 71: 15240. 182 403 EM; tested species: mouse

IHC

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Deng YP et al., J. Chem. Neuroanat. (2007)
4 ) Steiner P et al., EMBO J. (2005)
5 ) Nagy GG et al., J. Neurosci. (2004)
6 ) Passafaro M et al., Nature (2003)
7 ) Iihara K et al., J. Neurosci. (2001)
8 ) Daw MI et al., Neuron (2000)
9 ) Osten P et al., Neuron (1998)
10 ) He Y et al., Exp. Neurol. (1998)

Protocols

IHC

Other products for this target

GluA antibody - 182 403

References for GluA - 182 403