Synaptic Systems

Cat. No.: 168 003

Amount: 50 µg

Price: $375.00

Cat. No. 168 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)
Reactivity	Reacts with: rat (P23640, Q99P74), mouse (Q9ERI2, Q99P58). Other species not tested yet.
Specificity	Recognizes both isoforms rab 27A and B; no cross reaction to other rab proteins.
Matching control protein/peptide	168-0P
Data sheet	168_003.pdf

References for Rab27 - 168 003

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.
Maschalidi S, Nunes-Hasler P, Nascimento CR, Sallent I, Lannoy V, Garfa-Traore M, Cagnard N, Sepulveda FE, Vargas P, Lennon-Duménil AM, van Endert P, et al.
Nature communications (2017) 81: 1640. 168 003 WB; tested species: mouse

Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes.
de Wit H, Walter AM, Milosevic I, Gulyás-Kovács A, Riedel D, Sørensen JB, Verhage M
Cell (2009) 1385: 935-46. 168 003 WB

Phosphorylation negatively regulates exosome mediated secretion of cryAB in glioma cells.
Kore RA, Abraham EC
Biochimica et biophysica acta (2016) 18632: 368-77. 168 003 ICC

Cat. No.: 168 003

Amount: 50 µg

Price: $375.00

Phosphorylation negatively regulates exosome mediated secretion of cryAB in glioma cells.
Kore RA, Abraham EC
Biochimica et biophysica acta (2016) 18632: 368-77. 168 003 ICC

Background

Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.

1 ) Mahoney TR et al., Mol. Biol. Cell (2006)
2 ) Jordens I et al., Pigment Cell Res. (2006)
3 ) Tsuboi T et al., J. Cell. Sci. (2006)
4 ) Aslan D et al., Pediatr Hematol Oncol ()
5 ) Izumi T et al., Meth. Enzymol. (2005)
6 ) Kasai K et al., J. Clin. Invest. (2005)
7 ) Aizawa T et al., J. Clin. Invest. (2005)
8 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
9 ) Westbroek W et al., Pigment Cell Res. (2004)
10 ) Imai A et al., J. Cell. Sci. (2004)

Protocols

Standard protocol

OTHER

Preabsorbtion

Rab27 antibody - 168 003

References for Rab27 - 168 003